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GELSOLIN Antibody (OAAD00157)

100 ug
$255.00
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Gene Symbol:
GSN
NCBI Gene Id:
2934
Protein Name:
Gelsolin
Swissprot Id:
P06396
Alias Symbols:
ADF, AGEL,
Replacement Item:
This antibody may replace item sc-126909 from Santa Cruz Biotechnology.
Description of Target:
Gelsolin, a protein of leukocytes, platelets, and other cells, severs actin filaments in the presence of submicromolar calcium, thereby solating cytoplasmic actin gels. A gelsolin variant with 23 more N-terminal amino acids is a plasma component probably involved in the clearance of actin, the most abundant human protein, from the circulation. Gelsolin is located in 9q34. Plasma and cytoplasmic gelsolins are encoded by a single gene and contain a duplicated actin-binding domain
Host:
Mouse
Clonality:
Monoclonal
Purification:
Purified by the goat anti-mouse IgG Affinity chromatography.
Application:
WB, IHC, IP
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express GSN.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express GSN.
Immunogen:
Human plasma gelsolin.
Predicted Species Reactivity:
Human, Rabbit, bovine
Reconstitution and Storage:
At -20C for one year. After reconstitution, at 4C for one month. It can also be aliquotted and stored frozen at -20C for longer time.
Datasheets/Manuals:
Printable datasheet for GELSOLIN Antibody (OAAD00157) - OAAD00157
Specificity:
Human, rabbit, bovine.
No cross reactivity with other proteins.
Formulation:
Lyophilized from 1.2% sodium acetate, with 2mg BSA and 0.01mg NaN3 as preservative.
Clone:
GEL-42
Isotype:
IgG1
Application Info:
Western blot At 0.5-1ug/ml with the appropriate system to detect gelsolin in cells and tissues.
Immunohistochemistry(P) At 1-2ug/ml to detect gelsolin in formalin fixed and paraffin embedded tissues.
Immunohistochemistry(F) At1-2ug/ml to detect gelsolin in formalin or acetone fixed tissues.
Other applications have not been tested.
Optimal dilutions should be determined by end user.
Storage Buffer:
1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.
Target Reference:
1. Kwiatkowski, D. J.; Ozelius, L.; Schuback, D.; Gusella, J.; Breakefield, X. O. : The gelsolin (GSN) cDNA clone, from 9q32-34, identifies BclI and StuI RFLPs. Nucleic Acids Res. 17: 4425 only, 1989.
2. Kwiatkowski, D. J.; Westbrook, C. A.; Bruns, G. A. P.; Morton, C. C. : Localization of gelsolin proximal to ABL on chromosome 9. Am. J. Hum. Genet. 42: 565-572, 1988.
3. Kwiatkowski, D. J.; Stossel, T. P.; Orkin, S. H.; Mole, J. E.; Colten, H. R.; Yin, H. L. : Plasma and cytoplasmic gelsolins are encoded by a single gene and contain a duplicated actin-binding domain.
Nature 323: 455-458, 1986.

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