- NCBI Gene Id:
- Protein Name:
- Alias Symbols:
- ADF, AGEL,
- Replacement Item:
- This antibody may replace item sc-126909 from Santa Cruz Biotechnology.
- Description of Target:
- Gelsolin, a protein of leukocytes, platelets, and other cells, severs actin filaments in the presence of submicromolar calcium, thereby solating cytoplasmic actin gels. A gelsolin variant with 23 more N-terminal amino acids is a plasma component probably involved in the clearance of actin, the most abundant human protein, from the circulation. Gelsolin is located in 9q34. Plasma and cytoplasmic gelsolins are encoded by a single gene and contain a duplicated actin-binding domain
- Purified by the goat anti-mouse IgG Affinity chromatography.
- WB, IHC, IP
- Tissue Tool:
- Find tissues and cell lines supported by DNA array analysis to express GSN.
- RNA Seq:
- Find tissues and cell lines supported by RNA-seq analysis to express GSN.
- Human plasma gelsolin.
- Predicted Species Reactivity:
- Human, Rabbit, bovine
- Reconstitution and Storage:
- At -20C for one year. After reconstitution, at 4C for one month. It can also be aliquotted and stored frozen at -20C for longer time.
- Printable datasheet for GELSOLIN Antibody (OAAD00157) - OAAD00157
- Human, rabbit, bovine.
No cross reactivity with other proteins.
- Lyophilized from 1.2% sodium acetate, with 2mg BSA and 0.01mg NaN3 as preservative.
- Application Info:
- Western blot At 0.5-1ug/ml with the appropriate system to detect gelsolin in cells and tissues.
Immunohistochemistry(P) At 1-2ug/ml to detect gelsolin in formalin fixed and paraffin embedded tissues.
Immunohistochemistry(F) At1-2ug/ml to detect gelsolin in formalin or acetone fixed tissues.
Other applications have not been tested.
Optimal dilutions should be determined by end user.
- Storage Buffer:
- 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.
- Target Reference:
- 1. Kwiatkowski, D. J.; Ozelius, L.; Schuback, D.; Gusella, J.; Breakefield, X. O. : The gelsolin (GSN) cDNA clone, from 9q32-34, identifies BclI and StuI RFLPs. Nucleic Acids Res. 17: 4425 only, 1989.
2. Kwiatkowski, D. J.; Westbrook, C. A.; Bruns, G. A. P.; Morton, C. C. : Localization of gelsolin proximal to ABL on chromosome 9. Am. J. Hum. Genet. 42: 565-572, 1988.
3. Kwiatkowski, D. J.; Stossel, T. P.; Orkin, S. H.; Mole, J. E.; Colten, H. R.; Yin, H. L. : Plasma and cytoplasmic gelsolins are encoded by a single gene and contain a duplicated actin-binding domain.
Nature 323: 455-458, 1986.