GDNF ELISA Kit (Rat) (OKBB00153)

Catalog No: OKBB00153

Size:96 Tests

Rat GDNF ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva's rat GDNF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for GDNF has been precoated onto 96-well plates. Standards(NS0, S78-I211) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for GDNF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the rat GDNF amount of sample captured in plate.
• ELISA Kit Range: 31.2 pg/ml - 2,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  Mean (pg/mL)	155	624	1316	148	747	1324
  St. Dev.	6.98	26.21	72.38	10.06	59.01	102
  %CV	4.50	4.20	5.5	6.79	7.89	7.70

• ELISA Kit Component:
  • Lyophilized recombinant rat GDNF standard: 10 ng/tube x 2
  • Anti-rat GDNF Antibody Well Plate: 96 Wells
  • Sample diluent buffer: 30 mL
  • Biotinylated anti-rat GDNF antibody: 130 uL, dilution 1:100
  • Antibody diluent buffer: 12 mL
  • Avidin-Biotin-Peroxidase Complex (ABC): 130 uL, dilution 1:100
  • ABC diluent buffer: 12 mL
  • TMB color developing agent: 10 mL
  • TMB stop solution: 10 mL
• Additional Information: Range: 31.2pg/ml-2000pg/ml
  Notes: 1. To inspect the validity of experiment operation and the appropriateness of sample dilution proportion, pilot
  experiment using standards and a small number of samples is recommended.
  2. The TMB Color Developing agent is colorless and transparent before using, contact us freely if it is not the case.
  3. Before using the Kit, spin tubes and bring down all components to the bottom of tubes.
  4. Duplicate well assay is recommended for both standard and sample testing.
  5. Don’t let 96-well plate dry, for dry plate will inactivate active components on plate.
  6. Don’t reuse tips and tubes to avoid cross contamination.
  7. To avoid to use the reagents from different batches together.
  8. In order to avoid marginal effect of plate incubation due to temperature difference ( reaction may be stronger in the
  marginal wells), it is suggested that the diluted ABC and TMB solution will be pre-warmed in 37C for 30 min before
  using.
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: cell culture supernates, serum and plasma (heparin, EDTA)
• Sensitivity: <4 pg/ml
• Reference: 1. Lin, L.-F. H.; Doherty, D. H.; Lile, J. D.; Bektesh, S.; Collins, F. GDNF: a glial cell line-derived neurotrophic factor for
  midbrain dopaminergic neurons. Science 260: 1130-1132, 1993.
  2. Bermingham, N.; Hillermann, R.; Gilmour, F.; Martin, J. E.; Fisher, E. M. Human glial cell line-derived neurotrophic
  factor (GDNF) maps to chromosome 5. Hum. Genet. 96: 671-673, 1995.
  3. Durbec, P.; Marcos-Gutierrez, C. V.; Kilkenny, C.; Grigoriou, M.; Wartiowaara, K.; Suvanto, P.; Smith, D.; Ponder, B.;
  Costantini, F.; Saarma, M.; Sariola, H.; Pachnis, V. GDNF signalling through the Ret receptor tyrosine kinase.
  Nature 381: 789-793, 1996.
• Specificity: Natural and recombinant rat GDNF
• Immunogen: Expression system for standard: NS0; Immunogen sequence: S78-I211
• Description: For quantitative detection of rat GDNF in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Target Info

• Gene Symbol: GDNF
• Gene Full Name: glial cell derived neurotrophic factor
• Alias Symbols: Astrocyte derived trophic factor, ATF, ATF1, ATF2, Glial cell line derived neurotrophic factor, HFB1 GDNF, hGDNF, HSCR3
• NCBI Gene Id: 25453
• Protein Name: Glial cell line-derived neurotrophic factor
• Description of Target: Glial cell line-derived neurotrophic factor (GDNF) is a glycosylated, disulfide-bonded homodimer that is a distantly related member of the transforming growth factor-beta superfamily.1 GDNF, is a potent neurotrophic factor that promotes the survival of dopaminergic neurones in cultures including embryonic neuronal cultures.2 GDNF, in addition to its potential role in the differentiation and survival of central nervous system neurons, has profound effects on kidney organogenesis and the development of the peripheral nervous system.3 GDNF may have utility in the treatment of Parkinson's disease, which is marked by progressive degeneration of midbrain dopaminergic neurons.1 GDNF lies on the short arm of Rat chromosome 5, at 5p13.1-p13.3 ability to promote dopamine uptake in midbrain cultures.2 The standard product used in this kit is recombinant rat GDNF, which is a dimmer composed of two chains with 134 amino acids.
• Uniprot ID: Q07731
• Protein Accession #: NP_062012.1
• Nucleotide Accession #: NM_019139.1