GDI1 ELISA Kit (Human) (OKCD02566)

Catalog No: OKCD02566

Size:96WELLS

Human GDI1 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to GDP Dissociation Inhibitor 1 (GDI1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to GDP Dissociation Inhibitor 1 (GDI1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GDP Dissociation Inhibitor 1 (GDI1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of GDP Dissociation Inhibitor 1 (GDI1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level GDP Dissociation Inhibitor 1 (GDI1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level GDP Dissociation Inhibitor 1 (GDI1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-GDI1 Microplate: 96 Wells (12 x 8 Well strips)
  • GDI1 Lyophilized Standard: 2
  • 100X Biotinylated GDI1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates and other biological fluids
• Sensitivity: 0.052 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of GDP Dissociation Inhibitor 1 (GDI1). No significant cross-reactivity or interference between GDP Dissociation Inhibitor 1 (GDI1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: GDI1
• Gene Full Name: GDP dissociation inhibitor 1
• Alias Symbols: 1A;GDI-1;GDIL;guanosine diphosphate dissociation inhibitor 1;mental retardation, X-linked 48;MRX41;MRX48;oligophrenin-2;OPHN2;protein XAP-4;rab GDI alpha;rab GDP dissociation inhibitor alpha;rab GDP-dissociation inhibitor, alpha;RABGD1A;RABGDIA;testis secretory sperm-binding protein Li 208a;XAP-4;XLID41.
• NCBI Gene Id: 2664
• Protein Name: Rab GDP dissociation inhibitor alpha
• Description of Target: Regulates the GDP/GTP exchange reaction of most Rab proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. Promotes the dissociation of GDP-bound Rab proteins from the membrane and inhibits their activation. Promotes the dissociation of RAB1A, RAB3A, RAB5A and RAB10 from membranes.
• Uniprot ID: P31150
• Protein Accession #: NP_001484.1
• Nucleotide Accession #: NM_001493.2
• Protein Size (# AA): 447