GDF10 ELISA Kit (Mouse) (OKEH03609)

Catalog No: OKEH03609

Size:96T

Mouse GDF10 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Mouse, Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology GDF10 ELISA Kit (Mouse) (OKEH03609) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human Gdf10 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Gdf10 and incubated. The Gdf10 found in the sample or standards competes with the biotinylated Gdf10 for limited binding sites on the immobilized anti-human Gdf10 antibody. Excess unbound biotinylated Gdf10 and sample or standard Gdf10 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Gdf10 captured in the well and inversely proportional to the amount of Gdf10 which was contained in the sample or standard.
• ELISA Kit Range: 0.312-20ng/ml
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 82%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <= 4.6% (n = 20)
  Mean Inter-assay CV%: <= 7.7% (n = 20)
• ELISA Kit Component:
  • Anti-Gdf10 Microplate: 96 Wells (12 x 8 Well strips)
  • Gdf10 Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Gdf10-Biotin Complex: 1 x 60 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Biotin Complex Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.156 ng/mL
• Specificity: Natural and recombinant Mouse Bone morphogenetic protein 3B
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Gdf10
• Gene Full Name: growth differentiation factor 10
• Alias Symbols: Bmp3;Bmp3b;bone morphogenetic protein 3b;growth/differentiation factor 10.
• NCBI Gene Id: 14560
• Protein Name: P97737
• Description of Target: Growth/differentiation factor 10
• Uniprot ID: https://www.uniprot.org/uniprot/P97737
• Protein Accession #: NP_665684.2
• Nucleotide Accession #: NM_145741.2
• Protein Size (# AA): 476
• Molecular Weight: 53 kDa