GDA ELISA Kit (Human) (OKCD01044)

Catalog No: OKCD01044 (Formerly GWB-KBBKX5)

Size:96WELLS

Human GDA ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	95-103%	88-105%	95-105%	87-101%
  EDTA plasma(n=5)	94-101%	94-101%	92-104%	82-92%
  heparin plasma(n=5)	89-98%	85-93%	97-105%	87-94%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Guanine Deaminase (GDA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Guanine Deaminase (GDA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Guanine Deaminase (GDA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Guanine Deaminase (GDA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	86-94	91
  EDTA plasma(n=5)	93-101	97
  heparin plasma(n=5)	95-103	101

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Guanine Deaminase (GDA) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Guanine Deaminase (GDA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-GDA Microplate: 96 Wells (12 x 8 Well Strips)
  • GDA Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated GDA Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 0.129 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Guanine Deaminase (GDA). No significant cross-reactivity or interference between Guanine Deaminase (GDA) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: GDA
• Gene Full Name: guanine deaminase
• Alias Symbols: CYPIN;cytoplasmic PSD95 interactor;cytoplasmic PSD-95 interactor;GAH;GUANASE;guanine aminase;guanine aminohydrolase;guanine deaminase;NEDASIN;p51-nedasin.
• NCBI Gene Id: 9615
• Protein Name: Guanine deaminase
• Description of Target: Catalyzes the hydrolytic deamination of guanine, producing xanthine and ammonia.
• Uniprot ID: Q9Y2T3
• Protein Accession #: NP_001229434
• Nucleotide Accession #: NM_001242505.2
• Protein Size (# AA): 454