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Catalog No: OKCD07080
Size:96WELLS
Price: $750.00
SKU
OKCD07080
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Datasheets/ManualsPrintable datasheet for GCLM ELISA Kit (Human) (OKCD07080)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutamate Cysteine Ligase, Modifier Subunit (GCLM). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glutamate Cysteine Ligase, Modifier Subunit (GCLM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutamate Cysteine Ligase, Modifier Subunit (GCLM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Glutamate Cysteine Ligase, Modifier Subunit (GCLM) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glutamate Cysteine Ligase, Modifier Subunit (GCLM) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glutamate Cysteine Ligase, Modifier Subunit (GCLM) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
GCLM Microplate96 Wells (12 x 8 Well strips)
GCLM Lyophilized Standard2
100X GCLM HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 2 mL
Stop Solution1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.057ng/mL
Gene SymbolGCLM
Gene Full Nameglutamate-cysteine ligase modifier subunit
Alias Symbolsgamma-ECS regulatory subunit;gamma-glutamylcysteine synthetase regulatory subunit;GCS light chain;GLCLR;glutamate-cysteine ligase (gamma-glutamylcysteine synthetase), regulatory (30.8kD);Glutamate--cysteine ligase modifier subunit;glutamate-cysteine ligase modifier subunit delta2 alternative splicing;glutamate-cysteine ligase regulatory protein;glutamate--cysteine ligase regulatory subunit;GSC light chain.
NCBI Gene Id2730
Protein NameGlutamate--cysteine ligase regulatory subunit
Description of TargetGlutamate--cysteine ligase regulatory subunit
Uniprot IDP48507
Protein Accession #NP_001295182.1
Nucleotide Accession #NM_001308253.1
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