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- Phone: 858-552-6979
- Email: info@avivasysbio.com
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Printable datasheet for OALA02549 |
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Tested Species Reactivity | Rat |
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Predicted Species Reactivity | Mouse, Bovine, Rat, Human, Monkey |
Product Format | Liquid PBS (pH 7.4) with 0.2% BSA and 0.09% sodium azide |
Clonality | Polyclonal |
Host | Rabbit |
Conjugation | Unconjugated |
Application | ELISA, IHC, IHC-P, IP, WB |
Additional Information | Antigen Type: Synthetic peptide |
Reconstitution and Storage | Store at 4C or -20C. Avoid freeze-thaw cycles. |
Immunogen | A synthetic peptide derived from corresponding to aa 295-313 of rat GCLC (heavy subunit of GCS). |
Purification | Protein A purified |
Concentration | 1 mg/ml |
Specificity | Recognizes the heavy subunit of rat GCS. Species cross-reactivity: human, monkey and mouse. Cross-reacts weakly with bovine. |
Epitope | aa295-313 |
Warning | For research use only. |
Application Info | ELISA, IHC, IHC-P (5 ug/ml), IP, WB |
Application Data | Immunohistochemistry: OALA02549 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for OALA02549 was determined to be 5 ug/ml. Suitable for use in Western Blot and Immunoprecipitation. Western Blot: 2.5-5 ug/ml for 2hrs at RT. Immunoprecipitation: 10 ug/mg protein lysate. Denatured verified. Use Protein A. Positive control: Jurkat, MAD109, PC12 cells. |
Storage Buffer | PBS, pH 7.4, 0.09% Sodium Azide, 0.2% BSA |
Protein Range | aa295-313 |
Gene Symbol | GCLC |
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Alias Symbols | GCLC, GCL, GCS heavy chain, Gamma-ECS, GCS, GLCL, GLCLC |
NCBI Gene Id | 2729 |
Uniprot ID | P48506 |
Protein Accession # | NP_001489.1 |
Nucleotide Accession # | NM_001498 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "GCLC Antibody (OALA02549)"?
The tested species reactivity for this item is "Rat". This antibody is predicted to have homology to "Mouse, Bovine, Rat, Human, Monkey".
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How long will it take to receive "GCLC Antibody (OALA02549)"?
This item is available "Domestic: within 1 week delivery | International: 1 week".
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What buffer format is "GCLC Antibody (OALA02549)" provided in?
This item is provided in "Liquid PBS (pH 7.4) with 0.2% BSA and 0.09% sodium azide".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "GCLC Antibody (OALA02549)"?
This target may also be called "GCLC, GCL, GCS heavy chain, Gamma-ECS, GCS, GLCL, GLCLC" in publications.
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What is the shipping cost for "GCLC Antibody (OALA02549)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "GCLC Antibody (OALA02549)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "GCLC Antibody (OALA02549)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "GCLC Antibody (OALA02549)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "GCLC"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "GCLC"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "GCLC"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "GCLC"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "GCLC"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "GCLC"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.