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GCLC Antibody (OABB00446)

100 ug
$375.00
In Stock
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Predicted Species Reactivity:
Human, Mouse, Rat
Product Format:
Lyophilized
Clonality:
Polyclonal
Application:
WB, IHC-P
Additional Information:
Content: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3
:::
Background: GCLC, Glutamate--cysteine ligase catalytic subunit, is an enzyme that in humans is encoded by the GCLC gene.Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. The gene encoding the catalytic subunit encodes a protein of 367 amino acids with a calculated molecular weight of 72.773 kDa and maps to chromosome 6p12.1. Deficiency of gamma-glutamylcysteine synthetase in human is associated with enzymopathic hemolytic anemia.
Reconstitution and Storage:
0.2ml of distilled water will yield a concentration of 500ug/ml. At -20C for one year. After reconstitution, at 4C for one month. It can
also be aliquotted and stored frozen at -20C for a longer time.
Avoid repeated freezing and thawing.
Gene Symbol:
GCLC
Official Gene Full Name:
Glutamate--cysteine ligase catalytic subunit
NCBI Gene Id:
2729
Protein Name:
Glutamate--cysteine ligase catalytic subunit
Swissprot Id:
P48506
Protein Accession #:
NP_001184044.1; NP_001489.1
Nucleotide Accession #:
NM_001197115.1; NM_001498.3
Replacement Item:
This antibody may replace item sc-115522 from Santa Cruz Biotechnology.
Purification:
Immunogen affinity purified.
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express GCLC.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express GCLC.
Immunogen:
A synthetic peptide corresponding to a sequence at the N-terminal ofhuman GCS/GCLC, different from the related rat and mousesequences by one amino acid.
Predicted Homology Based on Immunogen Sequence:
Human; Mouse; Rat
Datasheets/Manuals:
Printable datasheet for anti-GCLC (OABB00446) antibody
Specificity:
Rabbit IgG
Target Reference:
1. Gipp, J. J., Chang, C., Mulcahy, R. T.Cloning and nucleotide sequence of a full-length cDNA for
human liver gamma-glutamylcysteine synthetase.Biochem. Biophys. Res. Commun. 185: 29-35,
1992.
2. Sierra-Rivera, E., Summar, M. L., Dasouki, M., Krishnamani, M. R. S., Phillips, J. A., Freeman, M.
L.Assignment of the gene (GLCLC) that encodes the heavy subunit of gamma-glutamylcysteine
synthetase to human chromosome 6.Cytogenet. Cell Genet. 70: 278-279, 1995.

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