GCLC Antibody (OABB00446)

Catalog#: OABB00446

• Size: 100 ug

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Lyophilized
• Clonality: Polyclonal
• Application: WB, IHC-P
• Additional Information: Content: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg Thimerosal, 0.05mg NaN₃
  Background: GCLC, Glutamate--cysteine ligase catalytic subunit, is an enzyme that in humans is encoded by the GCLC gene.Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. The gene encoding the catalytic subunit encodes a protein of 367 amino acids with a calculated molecular weight of 72.773 kDa and maps to chromosome 6p12.1. Deficiency of gamma-glutamylcysteine synthetase in human is associated with enzymopathic hemolytic anemia.
• Reconstitution and Storage: 0.2ml of distilled water will yield a concentration of 500ug/ml. At -20C for one year. After reconstitution, at 4C for one month. It can
  also be aliquotted and stored frozen at -20C for a longer time.
  Avoid repeated freezing and thawing.
• Gene Symbol: GCLC
• Official Gene Full Name: Glutamate--cysteine ligase catalytic subunit
• NCBI Gene Id: 2729
• Protein Name: Glutamate--cysteine ligase catalytic subunit
• Swissprot Id: P48506
• Protein Accession #: NP_001184044.1; NP_001489.1
• Nucleotide Accession #: NM_001197115.1; NM_001498.3
• Replacement Item: This antibody may replace item sc-115522 from Santa Cruz Biotechnology.
• Purification: Immunogen affinity purified.
• RNA Seq: Find tissues and cell lines supported by RNA-seq analysis to express GCLC.
• Immunogen: A synthetic peptide corresponding to a sequence at the N-terminal ofhuman GCS/GCLC, different from the related rat and mousesequences by one amino acid.
• Predicted Homology Based on Immunogen Sequence: Human; Mouse; Rat
• Specificity: Rabbit IgG
• Target Reference: 1. Gipp, J. J., Chang, C., Mulcahy, R. T.Cloning and nucleotide sequence of a full-length cDNA for
  human liver gamma-glutamylcysteine synthetase.Biochem. Biophys. Res. Commun. 185: 29-35,
  1992.
  2. Sierra-Rivera, E., Summar, M. L., Dasouki, M., Krishnamani, M. R. S., Phillips, J. A., Freeman, M.
  L.Assignment of the gene (GLCLC) that encodes the heavy subunit of gamma-glutamylcysteine
  synthetase to human chromosome 6.Cytogenet. Cell Genet. 70: 278-279, 1995.

Protocol:

Tips Information: