GCLC Antibody (OABB00446)

Catalog No: OABB00446 (Formerly GWB-BBP369)

Size:100UG

Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Kidney Tissue Lysate
Lane 3: Rat Heart Tissue Lysate
Lane 1: Rat Lung Tissue Lysate

Product Info

• Tested Species Reactivity: Human, Rat
• Predicted Species Reactivity: Bovine|Human|Mouse|Rat
• Product Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Clonality: Polyclonal
• Clone: Polyclonal
• Isotype: Rabbit IgG
• Application: Immunohistochemistry|Western blot
• Additional Information: Notes: Tested Species: In-house tested species with positive results.
  Predicted Species: Species predicted to be fit for the product based on sequence similarities.
  By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
  Other applications have not been tested.
  Optimal dilutions should be determined by end users.
  
  Background: GCLC, Glutamate--cysteine ligase catalytic subunit, is an enzyme that in humans is encoded by the GCLC gene. Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. The gene encoding the catalytic subunit encodes a protein of 367 amino acids with a calculated molecular weight of 72.773 kDa and maps to chromosome 6p12.1. Deficiency of gamma-glutamylcysteine synthetase in human is associated with enzymopathic hemolytic anemia.
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Immunogen: Polypeptide
• Purification: Affinity Purified
• Concentration: 500 ug/ml
• Specificity: No cross reactivity with other proteins.
• Application Info: Western blot: 0.1-0.5 ug/ml: Rat: Human, Mouse
  Immunohistochemistry (Paraffin-embedded Section): 0.5-1 ug/ml: Human, Rat: Mouse: By Heat
• Reference: 1. Gipp, J. J., Chang, C., Mulcahy, R. T.Cloning and nucleotide sequence of a full-length cDNA for
  human liver gamma-glutamylcysteine synthetase.Biochem. Biophys. Res. Commun. 185: 29-35,
  1992.
  2. Sierra-Rivera, E., Summar, M. L., Dasouki, M., Krishnamani, M. R. S., Phillips, J. A., Freeman, M.
  L.Assignment of the gene (GLCLC) that encodes the heavy subunit of gamma-glutamylcysteine
  synthetase to human chromosome 6.Cytogenet. Cell Genet. 70: 278-279, 1995.
• Storage Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Description: Rabbit IgG polyclonal antibody for Glutamate--cysteine ligase catalytic subunit(GCLC) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

Target Info

• Gene Symbol: GCLC
• Gene Full Name: glutamate-cysteine ligase catalytic subunit
• Alias Symbols: gamma-ECS;gamma-glutamylcysteine synthetase;GCL;GCS;GCS heavy chain;GLCL;GLCLC;glutamate--cysteine ligase catalytic subunit.
• NCBI Gene Id: 2729
• Protein Name: Glutamate--cysteine ligase catalytic subunit
• Description of Target: Glutamate--cysteine ligase catalytic subunit
• Uniprot ID: P48506
• Protein Accession #: NP_001184044.1; NP_001489.1
• Nucleotide Accession #: NM_001197115.1; NM_001498.3
• Molecular Weight: 72766 MW