- Predicted Species Reactivity:
- Human, Mouse, Rat
- Product Format:
- Lyophilized
- Clonality:
- Polyclonal
- Application:
- WB, IHC-P
- Additional Information:
- Content: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3
- :::
- Background: GCLC, Glutamate--cysteine ligase catalytic subunit, is an enzyme that in humans is encoded by the GCLC gene.Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. The gene encoding the catalytic subunit encodes a protein of 367 amino acids with a calculated molecular weight of 72.773 kDa and maps to chromosome 6p12.1. Deficiency of gamma-glutamylcysteine synthetase in human is associated with enzymopathic hemolytic anemia.
- Reconstitution and Storage:
- 0.2ml of distilled water will yield a concentration of 500ug/ml. At -20C for one year. After reconstitution, at 4C for one month. It can
also be aliquotted and stored frozen at -20C for a longer time.
Avoid repeated freezing and thawing.
- Official Gene Full Name:
- Glutamate--cysteine ligase catalytic subunit
- NCBI Gene Id:
- 2729
- Protein Name:
- Glutamate--cysteine ligase catalytic subunit
- Replacement Item:
- This antibody may replace item sc-115522 from Santa Cruz Biotechnology.
- Purification:
- Immunogen affinity purified.
- Tissue Tool:
- Find tissues and cell lines supported by DNA array analysis to express GCLC.

- RNA Seq:
- Find tissues and cell lines supported by RNA-seq analysis to express GCLC.

- Immunogen:
- A synthetic peptide corresponding to a sequence at the N-terminal ofhuman GCS/GCLC, different from the related rat and mousesequences by one amino acid.
- Predicted Homology Based on Immunogen Sequence:
- Human; Mouse; Rat
- Specificity:
- Rabbit IgG
- Target Reference:
- 1. Gipp, J. J., Chang, C., Mulcahy, R. T.Cloning and nucleotide sequence of a full-length cDNA for
human liver gamma-glutamylcysteine synthetase.Biochem. Biophys. Res. Commun. 185: 29-35,
1992.
2. Sierra-Rivera, E., Summar, M. L., Dasouki, M., Krishnamani, M. R. S., Phillips, J. A., Freeman, M.
L.Assignment of the gene (GLCLC) that encodes the heavy subunit of gamma-glutamylcysteine
synthetase to human chromosome 6.Cytogenet. Cell Genet. 70: 278-279, 1995.