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GCLC antibody - N-terminal region (ARP54576_P050)

100 ul
$319.00
In Stock

Conjugation Options

ARP54576_P050-FITC Conjugated

ARP54576_P050-HRP Conjugated

ARP54576_P050-Biotin Conjugated

Gene Symbol:
GCLC
NCBI Gene Id:
2729
Official Gene Full Name:
Glutamate-cysteine ligase, catalytic subunit
Protein Name:
Glutamate-cysteine ligase EMBL BAE97618.1
Swissprot Id:
P48506
Protein Accession #:
NP_001489
Nucleotide Accession #:
NM_001498
Alias Symbols:
GCS, GLCL, GLCLC, GCL
Replacement Item:
This antibody may replace item sc-115522 from Santa Cruz Biotechnology.
Description of Target:
Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. GCLC is the catalytic subunit of 637 amino acids with a calculated molecular weight of 72.773 kDa. Deficiency of gamma-glutamylcysteine synthetase in human is associated with enzymopathic hemolytic anemia.Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. The gene encoding the catalytic subunit encodes a protein of 367 amino acids with a calculated molecular weight of 72.773 kDa and maps to chromosome 6. The regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Deficiency of gamma-glutamylcysteine synthetase in human is associated with enzymopathic hemolytic anemia. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Protein Size (# AA):
637
Molecular Weight:
73kDa
Host:
Rabbit
Clonality:
Polyclonal
Purification:
Affinity Purified
Application:
WB, IHC
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express GCLC.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express GCLC.
Immunogen:
The immunogen is a synthetic peptide directed towards the N terminal region of human GCLC
Species Reactivity:
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish
Predicted Homology Based on Immunogen Sequence:
Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 93%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 79%
Complete computational species homology data:
Anti-GCLC (ARP54576_P050)
Peptide Sequence:
Synthetic peptide located within the following region: VLETLQEKGERTNPNHPTLWRPEYGSYMIEGTPGQPYGGTMSEFNTVEAN
Product Format:
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reconstitution and Storage:
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Concentration:
Batch dependent within range: 100 ul at 0.5 - 1 mg/ml
Protein Interactions:
UBC; PPID; GCLM; CCL22; PAXIP1; ELAVL1;
Blocking Peptide:
For anti-GCLC (ARP54576_P050) antibody is Catalog # AAP54576 (Previous Catalog # AAPP31360)
Datasheets/Manuals:
Printable datasheet for anti-GCLC (ARP54576_P050) antibody
Target Reference:
Jonsson,L.S., (2008) Int Arch Occup Environ Health 81 (7), 913-919
Publications:

Tomasi, M. L. et al. Molecular mechanisms of lipopolysaccharide-mediated inhibition of glutathione synthesis in mice. Free Radic. Biol. Med. 68, 148-58 (2014). WB, IHC, Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish 24296246

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