GCLC Antibody - middle region : HRP (ARP54577_P050-HRP)

Catalog No: ARP54577_P050-HRP

Size:100ul

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Yeast, Zebrafish
• Product Format: Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.
• Clonality: Polyclonal
• Host: Rabbit
• Conjugation: HRP: Horseradish Peroxidase
• Application: IHC, WB
• Reconstitution and Storage: All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human GCLC
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Yeast: 100%; Zebrafish: 100%
• Peptide Sequence: Synthetic peptide located within the following region: RISKSRYDSIDSYLSKCGEKYNDIDLTIDKEIYEQLLQEGIDHLLAQHVA
• Concentration: 0.5 mg/ml
• Blocking Peptide: For anti-GCLC (ARP54577_P050-HRP) antibody is Catalog # AAP54577 (Previous Catalog # AAPP44516)
• Publications:

  Bardag-Gorce, F. et al. Proteasome inhibitor up regulates liver antioxidative enzymes in rat model of alcoholic liver disease. Exp. Mol. Pathol. 90, 123-30 (2011). WB, Human, Yeast, Dog, Pig, Horse, Rabbit, Rat, Guinea pig, Mouse, Bovine, Zebrafish 21036165

  Ramani, K., Tomasi, M. L., Yang, H., Ko, K. & Lu, S. C. Mechanism and significance of changes in glutamate-cysteine ligase expression during hepatic fibrogenesis. J. Biol. Chem. 287, 36341-55 (2012). WB, Human, Yeast, Dog, Pig, Horse, Rabbit, Rat, Guinea pig, Mouse, Bovine, Zebrafish 22942279

Target Info

• Gene Symbol: GCLC
• Gene Full Name: Glutamate-cysteine ligase, catalytic subunit
• Alias Symbols: GCL, GCS, GLCL, GLCLC
• NCBI Gene Id: 2729
• Protein Name: Glutamate--cysteine ligase catalytic subunit
• Description of Target: Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. GCLC is the catalytic subunit of 637 amino acids with a calculated molecular weight of 72.773 kDa. Deficiency of gamma-glutamylcysteine synthetase in human is associated with enzymopathic hemolytic anemia.
• Uniprot ID: P48506
• Protein Accession #: NP_001489
• Nucleotide Accession #: NM_001498
• Protein Size (# AA): 637
• Molecular Weight: 73kDa
• Protein Interactions: UBC; PPID; GCLM; CCL22; PAXIP1; ELAVL1;