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GCLC Antibody - middle region (ARP54577_P050)

100 ul
$319.00
In Stock
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Conjugation Options

ARP54577_P050-FITC Conjugated

ARP54577_P050-HRP Conjugated

ARP54577_P050-Biotin Conjugated

Gene Symbol:
GCLC
Official Gene Full Name:
Glutamate-cysteine ligase, catalytic subunit
NCBI Gene Id:
2729
Protein Name:
Glutamate--cysteine ligase catalytic subunit
Swissprot Id:
P48506
Protein Accession #:
NP_001489
Nucleotide Accession #:
NM_001498
Alias Symbols:
GCL, GCS, GLCL, GLCLC
Replacement Item:
This antibody may replace item sc-115522 from Santa Cruz Biotechnology.
Description of Target:
Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. GCLC is the catalytic subunit of 637 amino acids with a calculated molecular weight of 72.773 kDa. Deficiency of gamma-glutamylcysteine synthetase in human is associated with enzymopathic hemolytic anemia.
Protein Size (# AA):
637
Molecular Weight:
73kDa
Host:
Rabbit
Clonality:
Polyclonal
Purification:
Affinity Purified
Application:
IHC, WB
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express GCLC.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express GCLC.
Immunogen:
The immunogen is a synthetic peptide directed towards the middle region of human GCLC
Predicted Species Reactivity:
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Yeast, Zebrafish
Tested Species Reactivity:
Human, Mouse
Predicted Homology Based on Immunogen Sequence:
Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Yeast: 100%; Zebrafish: 100%
Complete computational species homology data:
Anti-GCLC (ARP54577_P050)
Peptide Sequence:
Synthetic peptide located within the following region: RISKSRYDSIDSYLSKCGEKYNDIDLTIDKEIYEQLLQEGIDHLLAQHVA
Product Format:
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reconstitution and Storage:
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Concentration:
Batch dependent within range: 100 ul at 0.5 - 1 mg/ml
Protein Interactions:
UBC; PPID; GCLM; CCL22; PAXIP1; ELAVL1;
Blocking Peptide:
For anti-GCLC (ARP54577_P050) antibody is Catalog # AAP54577 (Previous Catalog # AAPP44516)
Datasheets/Manuals:
Printable datasheet for anti-GCLC (ARP54577_P050) antibody
Publications:

Bardag-Gorce, F. et al. Proteasome inhibitor up regulates liver antioxidative enzymes in rat model of alcoholic liver disease. Exp. Mol. Pathol. 90, 123-30 (2011). IHC, WB, Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Yeast, Zebrafish 21036165

Ramani, K., Tomasi, M. L., Yang, H., Ko, K. & Lu, S. C. Mechanism and significance of changes in glutamate-cysteine ligase expression during hepatic fibrogenesis. J. Biol. Chem. 287, 36341-55 (2012). IHC, WB, Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Yeast, Zebrafish 22942279

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