- Tested Species Reactivity:
- Human, Mouse
- Predicted Species Reactivity:
- Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Yeast, Zebrafish
- Product Format:
- Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
- Clonality:
- Polyclonal
- Host:
- Rabbit
- Application:
- IHC, WB
- Reconstitution and Storage:
- For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
- Gene Symbol:
- GCLC
- Official Gene Full Name:
- Glutamate-cysteine ligase, catalytic subunit
- NCBI Gene Id:
- 2729
- Protein Name:
- Glutamate--cysteine ligase catalytic subunit
- Swissprot Id:
- P48506
- Protein Accession #:
- NP_001489
- Nucleotide Accession #:
- NM_001498
- Alias Symbols:
- GCL, GCS, GLCL, GLCLC
- Replacement Item:
- This antibody may replace item sc-115522 from Santa Cruz Biotechnology.
- Description of Target:
- Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. GCLC is the catalytic subunit of 637 amino acids with a calculated molecular weight of 72.773 kDa. Deficiency of gamma-glutamylcysteine synthetase in human is associated with enzymopathic hemolytic anemia.
- Protein Size (# AA):
- 637
- Molecular Weight:
- 73kDa
- Purification:
- Affinity Purified
- Tissue Tool:
- Find tissues and cell lines supported by DNA array analysis to express GCLC.
- RNA Seq:
- Find tissues and cell lines supported by RNA-seq analysis to express GCLC.
- Immunogen:
- The immunogen is a synthetic peptide directed towards the middle region of human GCLC
- Predicted Homology Based on Immunogen Sequence:
- Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Yeast: 100%; Zebrafish: 100%
- Complete computational species homology data:
- Anti-GCLC (ARP54577_P050)
- Peptide Sequence:
- Synthetic peptide located within the following region: RISKSRYDSIDSYLSKCGEKYNDIDLTIDKEIYEQLLQEGIDHLLAQHVA
- Concentration:
- Batch dependent within range: 100 ul at 0.5 - 1 mg/ml
- Protein Interactions:
- UBC; PPID; GCLM; CCL22; PAXIP1; ELAVL1;
- Blocking Peptide:
- For anti-GCLC (ARP54577_P050) antibody is Catalog # AAP54577 (Previous Catalog # AAPP44516)
- Datasheets/Manuals:
- Printable datasheet for anti-GCLC (ARP54577_P050) antibody
- Publications:
Bardag-Gorce, F. et al. Proteasome inhibitor up regulates liver antioxidative enzymes in rat model of alcoholic liver disease. Exp. Mol. Pathol. 90, 123-30 (2011). IHC, WB, Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Yeast, Zebrafish 21036165
Ramani, K., Tomasi, M. L., Yang, H., Ko, K. & Lu, S. C. Mechanism and significance of changes in glutamate-cysteine ligase expression during hepatic fibrogenesis. J. Biol. Chem. 287, 36341-55 (2012). IHC, WB, Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Yeast, Zebrafish 22942279
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