GCLC Antibody - middle region (ARP54577_P050)

Catalog#: ARP54577_P050

• Size: 100 ul

• Gene Symbol: GCLC
• Official Gene Full Name: Glutamate-cysteine ligase, catalytic subunit
• NCBI Gene Id: 2729
• Protein Name: Glutamate--cysteine ligase catalytic subunit
• Swissprot Id: P48506
• Protein Accession #: NP_001489
• Nucleotide Accession #: NM_001498
• Alias Symbols: GCL, GCS, GLCL, GLCLC
• Replacement Item: This antibody may replace item sc-115522 from Santa Cruz Biotechnology.
• Description of Target: Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. GCLC is the catalytic subunit of 637 amino acids with a calculated molecular weight of 72.773 kDa. Deficiency of gamma-glutamylcysteine synthetase in human is associated with enzymopathic hemolytic anemia.
• Protein Size (# AA): 637
• Molecular Weight: 73kDa
• Host: Rabbit
• Clonality: Polyclonal
• Purification: Affinity Purified
• Application: IHC, WB
• RNA Seq: Find tissues and cell lines supported by RNA-seq analysis to express GCLC.
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human GCLC
• Predicted Species Reactivity: Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Yeast, Zebrafish
• Tested Species Reactivity: Human, Mouse
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Yeast: 100%; Zebrafish: 100%
• Peptide Sequence: Synthetic peptide located within the following region: RISKSRYDSIDSYLSKCGEKYNDIDLTIDKEIYEQLLQEGIDHLLAQHVA
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Concentration: Batch dependent within range: 100 ul at 0.5 - 1 mg/ml
• Protein Interactions: UBC; PPID; GCLM; CCL22; PAXIP1; ELAVL1;
• Blocking Peptide: For anti-GCLC (ARP54577_P050) antibody is Catalog # AAP54577 (Previous Catalog # AAPP44516)
• Publications:

  Bardag-Gorce, F. et al. Proteasome inhibitor up regulates liver antioxidative enzymes in rat model of alcoholic liver disease. Exp. Mol. Pathol. 90, 123-30 (2011). IHC, WB, Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Yeast, Zebrafish 21036165

  Ramani, K., Tomasi, M. L., Yang, H., Ko, K. & Lu, S. C. Mechanism and significance of changes in glutamate-cysteine ligase expression during hepatic fibrogenesis. J. Biol. Chem. 287, 36341-55 (2012). IHC, WB, Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Yeast, Zebrafish 22942279

Protocol:

• Reconstitution Instructions
• Immunohistochemistry (IHC) Protocol
• Immunocytochemistry (ICC) Protocol
• Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
• Western Blotting/Immunoblotting (WB/IB) Protocol
• Blocking Peptide Competition Protocol (BPCP)
• Immunoprecipitation (IP) Protocol

Tips Information:

• IHC Tips & Tricks
• ICC Tips & Tricks
• ELISA Tips & Tricks
• WB/IB Tips & Tricks
• IP Tips & Tricks

See our General FAQ page.

Product Protocols: GCLC antibody tested with Human Placenta Tissue (ARP54577_P050)