Catalog No: OKEH03746
Size:96T
Price: $675.00
SKU
OKEH03746
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology GCK ELISA Kit (Rat) (OKEH03746) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Gck has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Gck is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Gck captured in well.
ELISA Kit Range78-5000pg/mL
ELISA Kit Component
ComponentAmount
Gck Microplate96 Wells (12 x 8 Well strips)
Gck Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated Gck Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 10 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity39 pg/mL
SpecificityNatural and recombinant Rat Glucokinase
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolGck
Gene Full Nameglucokinase
Alias SymbolsGLK;glucokinase variant 3;GLUKA;hexokinase type IV;hexokinase-4;hexokinase-D;HK IV;HK4;RNGK2.
NCBI Gene Id24385
Protein NameP17712
Description of TargetGlucokinase
Uniprot IDhttps://www.uniprot.org/uniprot/P17712
Protein Accession #NP_001257779.1
Nucleotide Accession #NM_001270850.1
Protein Size (# AA)498
Molecular Weight55 kDa
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