GCK Chemi-Luminescent ELISA Kit (Human) (OKCD03502)

Catalog No: OKCD03502

Size:96WELLS

Human GCK ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	80-104%	95-103%	94-101%	96-104%
  EDTA Plasma (n=5)	99-105%	87-101%	92-105%	93-101%
  Heparin Plasma (n=5)	89-101%	78-98%	83-95%	93-101%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Glucokinase (GCK). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Glucokinase (GCK). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Glucokinase (GCK) level in the sample or standard.
• ELISA Kit Range: 0.21-150ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	93-101	96
  EDTA Plasma (n=5)	96-105	101
  Heparin Plasma (n=5)	94-102	97

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glucokinase (GCK) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glucokinase (GCK) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • GCK Microplate: 96 Wells (12 x 8 Well strips)
  • GCK Lyophilized Standard: 2
  • 100X GCK Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: 0.21 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: GCK
• Gene Full Name: glucokinase
• Alias Symbols: ATP:D-hexose 6-phosphotransferase;FGQTL3;GK;GLK;glucokinase (hexokinase 4);hexokinase D, pancreatic isozyme;hexokinase type IV;hexokinase-4;Hexokinase-D;HHF3;HK IV;HK4;HKIV;HXKP;LGLK;MODY2;PNDM1.
• NCBI Gene Id: 2645
• Protein Name: Glucokinase
• Description of Target: Catalyzes the initial step in utilization of glucose by the beta-cell and liver at physiological glucose concentration. Glucokinase has a high Km for glucose, and so it is effective only when glucose is abundant. The role of GCK is to provide G6P for the synthesis of glycogen. Pancreatic glucokinase plays an important role in modulating insulin secretion. Hepatic glucokinase helps to facilitate the uptake and conversion of glucose by acting as an insulin-sensitive determinant of hepatic glucose usage.
• Uniprot ID: P35557
• Protein Accession #: NP_000153
• Nucleotide Accession #: NM_000162