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| Datasheets/Manuals | Printable datasheet for GCC2 Antibody (OAEB00189) |
|---|
| Predicted Species Reactivity | Human|Rat |
|---|---|
| Product Format | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Clonality | Polyclonal |
| Host | Goat |
| Application | IHC |
| Reconstitution and Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| Immunogen | GCC2 |
| Purification | Ammonium Sulfate Precipitated; Affinity Purified |
| Peptide Sequence | C-QKLTNKNNKIED |
| Concentration | 0.5 mg/ml |
| Specificity | This antibody is expected to recognise both reported isoforms (NP_055450.1and NP_852118.1). |
| Application Info | P-ELISA: Antibody detection limit dilution 1:16000. WB: Preliminary experiments gave an approx 60kDa band in Human Lymph Node and Testis lysates after 0.03ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 185kDa according to NP_055450.1 and of 196kDa according to NP_852118.1. The 60kDa band was successfully blocked by incubation with the immunizing peptide. IHC: Paraffin embedded Human Spleen, Small Intestine, and Kidney. Recommended concentration: 3.75 ug/mL |
| Reference | * Efimov A, Kharitonov A, Efimova N, Loncarek J, Miller PM, Andreyeva N, Gleeson P, Galjart N, Maia AR, McLeod IX, Yates JR 3rd, Maiato H, Khodjakov A, Akhmanova A, Kaverina I.; Asymmetric CLASP-dependent nucleation of noncentrosomal microtubules at the trans-Golgi network.; Dev Cell. 2007 Jun;12(6):917-30.; Pubmed 17543864 |
| Gene Symbol | GCC2 |
|---|---|
| Gene Full Name | GRIP and coiled-coil domain containing 2 |
| Alias Symbols | 185 kDa Golgi coiled-coil protein;CLL-associated antigen KW-11;CTCL tumor antigen se1-1;GCC protein, 185-kD;GCC185;Golgi coiled-coil protein GCC185;GRIP and coiled-coil domain-containing protein 2;Ran-binding protein 2-like 4;RANBP2L4;REN53;renal carcinoma antigen NY-REN-53. |
| NCBI Gene Id | 9648 |
| Protein Name | GRIP and coiled-coil domain-containing protein 2 |
| Uniprot ID | Q8IWJ2 |
| Protein Accession # | NP_055450.1 |
| Molecular Weight | 185; 196 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "GCC2 Antibody (OAEB00189)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Rat".
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How long will it take to receive "GCC2 Antibody (OAEB00189)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "GCC2 Antibody (OAEB00189)" provided in?
This item is provided in "Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "GCC2 Antibody (OAEB00189)"?
This target may also be called "185 kDa Golgi coiled-coil protein;CLL-associated antigen KW-11;CTCL tumor antigen se1-1;GCC protein, 185-kD;GCC185;Golgi coiled-coil protein GCC185;GRIP and coiled-coil domain-containing protein 2;Ran-binding protein 2-like 4;RANBP2L4;REN53;renal carcinoma antigen NY-REN-53." in publications.
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What is the shipping cost for "GCC2 Antibody (OAEB00189)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "GCC2 Antibody (OAEB00189)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "GCC2 Antibody (OAEB00189)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "185; 196".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "GCC2 Antibody (OAEB00189)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "GCC2"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "GCC2"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "GCC2"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "GCC2"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "GCC2"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "GCC2"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.
