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Catalog No: OKCD03411
Size:96WELLS
Price: $800.00
SKU
OKCD03411
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GC Chemi-Luminescent ELISA Kit (Human) (OKCD03411)

Datasheets/ManualsPrintable datasheet for GC Chemi-Luminescent ELISA Kit (Human) (OKCD03411)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationChemiluminescent immunoassay
ELISA Kit Detection MethodChemiluminescent
ELISA Kit Duration2h, 40min
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)93-101%86-103%80-91%82-103%
EDTA Plasma (n=5)93-101%82-103%82-91%97-105%
Heparin Plasma (n=5)87-96%95-105%98-105%79-104%
ELISA Kit PrincipleThe microplate provided in this kit has been pre-coated with an antibody specific to Vitamin D Binding Protein (DBP). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Vitamin D Binding Protein (DBP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Vitamin D Binding Protein (DBP) level in the sample or standard.
ELISA Kit Range1.37-1,000ng/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)88-9794
EDTA Plasma (n=5)80-9083
Heparin Plasma (n=5)91-104101
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Vitamin D Binding Protein (DBP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Vitamin D Binding Protein (DBP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
GC Microplate96 Wells (12 x 8 Well strips)
GC Lyophilized Standard2
100X GC Biotinylated Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids
Sensitivity1.37 ng/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolGC
Gene Full NameGC vitamin D binding protein
Alias SymbolsDBP;DBP/GC;DBP-maf;epididymis secretory protein Li 51;gc protein-derived macrophage activating factor;gc-globulin;GcMAF;Gc-MAF;GRD3;Group-specific component;group-specific component (vitamin D binding protein);HEL-S-51;VDB;VDBG;VDBP;vitamin D-binding alpha-globulin;vitamin D-binding protein;vitamin D-binding protein-macrophage activating factor.
NCBI Gene Id2638
Protein NameVitamin D-binding protein
Description of TargetInvolved in vitamin D transport and storage, scavenging of extracellular G-actin, enhancement of the chemotactic activity of C5 alpha for neutrophils in inflammation and macrophage activation.
Uniprot IDP02774
Protein Accession #NP_000574
Nucleotide Accession #NM_000583
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