GBP1 ELISA Kit (Human) (OKCD00435)

Catalog No: OKCD00435 (Formerly GWB-KBBBV7)

Size:96WELLS

Human GBP1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	87-94%	79-105%	85-93%	86-93%
  EDTA plasma(n=5)	78-91%	93-105%	96-103%	90-99%
  heparin plasma(n=5)	89-103%	80-92%	83-97%	98-105%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Guanylate Binding Protein 1, Interferon Inducible (GBP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Guanylate Binding Protein 1, Interferon Inducible (GBP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Guanylate Binding Protein 1, Interferon Inducible (GBP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Guanylate Binding Protein 1, Interferon Inducible (GBP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	91-98	95
  EDTA plasma(n=5)	78-104	99
  heparin plasma(n=5)	96-103	99

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Guanylate Binding Protein 1, Interferon Inducible (GBP1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Guanylate Binding Protein 1, Interferon Inducible (GBP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-GBP1 Microplate: 96 Wells (12 x 8 Well Strips)
  • GBP1 Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated GBP1 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
• Sensitivity: < 0.066 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Guanylate Binding Protein 1, Interferon Inducible (GBP1). No significant cross-reactivity or interference between Guanylate Binding Protein 1, Interferon Inducible (GBP1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: GBP1
• Gene Full Name: guanylate binding protein 1
• Alias Symbols: GBP-1;GTP-binding protein 1;guanine nucleotide-binding protein 1;guanylate binding protein 1, interferon-inducible, 67kDa;guanylate-binding protein 1;huGBP-1;interferon-induced guanylate-binding protein 1.
• NCBI Gene Id: 2633
• Protein Name: Guanylate-binding protein 1
• Description of Target: Hydrolyzes GTP to GMP in 2 consecutive cleavage reactions. Exhibits antiviral activity against influenza virus. Promote oxidative killing and deliver antimicrobial peptides to autophagolysosomes, providing broad host protection against different pathogen classes.
• Uniprot ID: P32455
• Protein Accession #: NP_002044
• Nucleotide Accession #: NM_002053.2
• Protein Size (# AA): 592