Catalog No: OKWB00143
Size:96T
Price: $450.00
SKU
OKWB00143
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Datasheets/ManualsPrintable datasheet for GBD-1 ELISA Kit (Goat) (OKWB00143)
Product Info
Predicted Species ReactivityCapra hircus|Goat
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit Linearity
Sample Type1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)91-100%86-105%85-93%86-102%
EDTA Plasma (n=5)85-100%82-98%86-101%86-101%
Heparin Plasma (n=5)83-99%80-98%85-98%88-100%
ELISA Kit PrincipleAviva Systems Biology Gbd-1 ELISA Kit (Goat) (OKWB00143) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Gbd-1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Gbd-1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Gbd-1 captured in the well.
ELISA Kit Range78.125-5000pg/ml
ELISA Kit Recovery
Sample TypeRangeMean
Serum (n=5)85-10495
EDTA Plasma (n=5)90-10196
Heparin Plasma (n=5)85-10395
ELISA Kit ReproducibilityMean Intra-Assay Precision <10%
Mean Inter-Assay Precision <12%
ELISA Kit Component
ComponentAmount
Gbd-1 Microplate96 Wells (12 x 8 Well strips)
Gbd-1 Lyophilized Standard2 x 5 ng
100X Gbd-1 Biotinylated Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 20 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids
Sensitivity46.9 pg/mL
Assay InfoAssay Type: Quantitative Sandwich ELISA
Gene SymbolGBD-1
Protein NameBeta-defensin 1
Uniprot IDD3YJ56
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