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GBA Chemi-Luminescent ELISA Kit (Human) (OKCD03501)

96 Wells
$719.00
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Predicted Species Reactivity:
Human
Kit Detection Method:
Chemiluminescent, visible wavelength light
Kit Duration:
~ 3.5 Hours
Kit Linearity:
Sample1:21:41:81:16
Serum (n=5)88-104%96-105%95-103%78-92%
EDTA Plasma (n=5)85-96%84-92%98-105%90-103%
Heparin Plasma (n=5)90-97%94-101%86-101%86-95%
Kit Principle:
Aviva Systems Biology GBA Chemi-Luminescent ELISA Kit (Human) (OKCD03501) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for GBA has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for GBA is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of a luminol substrate which is catalyzed by the HRP to produce light emission. The light emission is read by a luminometer (or photo-multiplier equipped instrument) and the intensity of the emitted light is proportional to the amount of sample GBA
Kit Range:
2.74-2,000 pg/mL
Kit Recovery:
MatrixRecovery range (%)Average(%)
Serum (n=5)83-9893
EDTA Plasma (n=5)88-10195
Heparin Plasma (n=5)87-9591
Kit Reproducibility:
Intra-assay reproducibility was evaluated using 20 replicates of 3 samples representing low, middle and high level GBA. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level GBA using 8 replicates on each of 3 plates.
Intra-Assay CV <10%
Inter-Assay CV <12%
Kit Component:
ComponentAmount
GBA Microplate96 Wells (12 x 8 Well strips)
GBA Lyophilized Standard2
100X GBA Biotinylated Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and Storage:
Store as indicated in product manual.
Sample Type:
Serum, plasma, tissue homogenates and other biological fluids
Sensitivity:
< 0.99 pg/mL
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Assay Info:
Assay Methodology: Quantitative Sandwich ELISA
Gene Symbol:
GBA
Alias Symbols:
Acid beta-glucosidase, Alglucerase, Beta-glucocerebrosidase, D-glucosyl-N-acylsphingosine glucohydrolase, GBA1, GC, GCB, GLUC, Glucosylceramidase, Imiglucerase
NCBI Gene Id:
2629
Protein Name:
Glucosylceramidase
Swissprot Id:
P04062
Protein Accession #:
NP_000148
Nucleotide Accession #:
NM_000157
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express GBA.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express GBA.

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162/06/2019 18:58
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Sr Scientist

For 6 months I had been evaluating GBA ELISAs for protein  expression without success. 

As usually I would find ELISAs where the standard curve performs as expected but my protein was not detected.

I had confirmed my GBA expression by Western blot using R&D Systems MAB7410. This is the best Monoclonal Ab I have found after an exhaustive search.

I know I am making GBA but the ELISAs I had evaluated, and there  were several, all failed to detect my GBA or my recombinant GBA standard. I suspect that the Antibody used in these ELISAs are generated using peptides which is fine except they do no detect whole GBA protein. 

Until I found this assay!

Both this kit and their standard ELISAs work briliantly, I purchaed a recombinant GBA from R&D Systems (cat# 7410) and this kit as well as the standard ELISA they offer detects it and quantitates it within a reasonable accuracy from a known concentration. The assay is simple to use and anyone who is reasearching Gaucher diease should use this kit. 

I would encourage the Gaucher disease community to validate this assay as their standard as it is the only ELISAs format assay that detects whole GBA protein.

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