Size:96 Wells
$719.00
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SKU
OKCD03501
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GBA Chemi-Luminescent ELISA Kit (Human) (OKCD03501)

Catalog#: OKCD03501
Domestic: within 1-2 weeks delivery | International: 1-2 weeks
More Information
Predicted Species Reactivity Human
Kit Detection Method Chemiluminescent, visible wavelength light
Kit Duration ~ 3.5 Hours
Kit Linearity
Sample1:21:41:81:16
Serum (n=5)88-104%96-105%95-103%78-92%
EDTA Plasma (n=5)85-96%84-92%98-105%90-103%
Heparin Plasma (n=5)90-97%94-101%86-101%86-95%
Kit Principle Aviva Systems Biology GBA Chemi-Luminescent ELISA Kit (Human) (OKCD03501) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for GBA has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for GBA is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of a luminol substrate which is catalyzed by the HRP to produce light emission. The light emission is read by a luminometer (or photo-multiplier equipped instrument) and the intensity of the emitted light is proportional to the amount of sample GBA
Kit Range 2.74-2,000 pg/mL
Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)83-9893
EDTA Plasma (n=5)88-10195
Heparin Plasma (n=5)87-9591
Kit Reproducibility Intra-assay reproducibility was evaluated using 20 replicates of 3 samples representing low, middle and high level GBA. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level GBA using 8 replicates on each of 3 plates.
Intra-Assay CV <10%
Inter-Assay CV <12%
Kit Component
ComponentAmount
GBA Microplate96 Wells (12 x 8 Well strips)
GBA Lyophilized Standard2
100X GBA Biotinylated Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and Storage Store as indicated in product manual.
Sample Type Serum, plasma, tissue homogenates and other biological fluids
Sensitivity < 0.99 pg/mL
Datasheets/Manuals Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Gene Symbol GBA
Alias Symbols Acid beta-glucosidase, Alglucerase, Beta-glucocerebrosidase, D-glucosyl-N-acylsphingosine glucohydrolase, GBA1, GC, GCB, GLUC, Glucosylceramidase, Imiglucerase
NCBI Gene Id 2629
Protein Name Glucosylceramidase
Swissprot Id P04062
Protein Accession # NP_000148
Nucleotide Accession # NM_000157
Tissue Tool Find tissues and cell lines supported by DNA array analysis to express GBA.
RNA Seq Find tissues and cell lines supported by RNA-seq analysis to express GBA.
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