GATA5 ELISA Kit (Human) (OKCD06250)

Catalog No: OKCD06250

Size:96WELLS

GATA5 ELISA Kit (Human) (OKCD06250) Standard Curve

Product Info

• Predicted Species Reactivity: Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colormetric|Regular detection
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to GATA Binding Protein 5 (GATA5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to GATA Binding Protein 5 (GATA5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GATA Binding Protein 5 (GATA5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of GATA Binding Protein 5 (GATA5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level GATA Binding Protein 5 (GATA5) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level GATA Binding Protein 5 (GATA5) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • GATA5 Microplate: 96 Wells (12 x 8 Well strips)
  • GATA5 Lyophilized Standard: 2
  • 100X GATA5 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C, -20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids.
• Sensitivity: < 0.056ng/mL

Target Info

• Gene Symbol: GATA5
• Gene Full Name: GATA binding protein 5
• Alias Symbols: CHTD5, GATAS, bB379O24.1
• NCBI Gene Id: 140628
• Protein Name: Transcription factor GATA-5
• Description of Target: The protein encoded by this gene is a transcription factor that contains two GATA-type zinc fingers. The encoded protein is known to bind to hepatocyte nuclear factor-1alpha (HNF-1alpha), and this interaction is essential for cooperative activation of the intestinal lactase-phlorizin hydrolase promoter. In other organisms, similar proteins may be involved in the establishment of cardiac smooth muscle cell diversity.
• Uniprot ID: Q9BWX5
• Protein Accession #: NP_536721.1
• Nucleotide Accession #: NM_080473.4