Catalog No: OPCD03454
Price: $0.00
SKU
OPCD03454
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Datasheets/Manuals | Printable datasheet for GATA4 Recombinant Protein (OPCD03454) |
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Predicted Species Reactivity | Rat|Rattus norvegicus |
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Product Format | Freeze-dried Powder. PBS, pH7.4, containing 0.01% SKL, 5% Trehalose. |
Host | E.coli |
Conjugation | Unconjugated |
Application | Positive control|Sodium sodecyl sulfate - polyacrylamide gel electrophoresis|Western blot |
Additional Information | Endotoxin Level: < 1.0 EU per 1 ug (determined by the LAL method) Residues: Arg228-Ala440 Subcellular Location: Nucleus Isoelectric Point: 10.1 |
:: | Phenomenon Explanation: The possible reasons that the actual band size differs from the predicted are as follows: 1) Splice Variants: Alternative splicing may create different sized proteins from the same gene. 2) Relative charge: The composition of amino acids may affect the charge of the protein. 3) Post-translational modification: Phosphorylation, glycosylation, methylation, etc. 4) Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form. 5) Polymerization of the target protein: Dimerization, multimerization, etc. |
Reconstitution and Storage | 2°C to 8°C|-80°C |
Concentration | 150 ug/mL (prior to lyoph) |
Purity | > 95% |
Source | E.coli |
Tag | N-terminal His Tag |
Gene Symbol | Gata4 |
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Gene Full Name | GATA binding protein 4 |
Alias Symbols | DNA-binding protein GATA-GT2;GATA-binding factor 4;transcription factor GATA-4. |
NCBI Gene Id | 54254 |
Protein Name | Transcription factor GATA-4 |
Description of Target | Transcriptional activator that binds to the consensus sequence 5'-AGATAG-3' and plays a key role in cardiac development (By similarity). In cooperation with TBX5, it binds to cardiac super-enhancers and promotes cardiomyocyte gene expression, while it downregulates endocardial and endothelial gene expression (By similarity). Acts as a transcriptional activator of ANF in cooperation with NKX2-5 (By similarity). Promotes cardiac myocyte enlargement (By similarity). Required during testicular development (By similarity). Involved in bone morphogenetic protein (BMP)-mediated induction of cardiac-specific gene expression (PubMed:15329343). Binds to BMP response element (BMPRE) DNA sequences within cardiac activating regions (PubMed:15329343). May play a role in sphingolipid signaling by regulating the expression of sphingosine-1-phosphate degrading enzyme, spingosine-1-phosphate lyase (By similarity). |
Uniprot ID | P46152 |
Protein Accession # | NP_653331.1 |
Nucleotide Accession # | NM_144730.1 |
Protein Size (# AA) | Arg228~Ala440 |
Molecular Weight | 33kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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