GAS1 Antibody - N-terminal region (OAAB08110)

Catalog No: OAAB08110

Size:400ul

GAS1 Antibody (N-term) (Cat. #OAAB08110) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the GAS1 antibody detected the GAS1 protein (arrow).

GAS1 Antibody (N-term) (Cat. #OAAB08110)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GAS1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

GAS1 Antibody (N-term) (Cat. #OAAB08110) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Confocal immunofluorescent analysis of GAS1 Antibody (N-term) (Cat. #OAAB08110) with NCI-H460 cell followed by Alexa Fluor488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, IHC-P, IF, FC
• Additional Information: Function: Specific growth arrest protein involved in growth suppression. Blocks entry to S phase. Prevents cycling of normal and transformed cells
  Cellular Location: Cell membrane; Lipid-anchor, GPI-anchor
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This GAS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 95-127 amino acids from the N-terminal region of human GAS1.
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Intended Use: GAS1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
• Application Info: WB: 1:1000
  IHC-P: 1:50~100
  IF: 1:10~50
  FC: 1:10~50

Target Info

• Gene Symbol: GAS1
• Alias Symbols: Growth arrest-specific protein 1, GAS-1, GAS1
• NCBI Gene Id: 2619
• Protein Name: Growth arrest-specific protein 1
• Description of Target: Growth arrest-specific 1 plays a role in growth suppression. GAS1 blocks entry to S phase and prevents cycling of normal and transformed cells. Gas1 is a putative tumor suppressor gene.
• Uniprot ID: P54826
• Protein Accession #: NP_002039.2
• Molecular Weight: 35693 Da