GARS Antibody - middle region (OAGA04658)

Catalog No: OAGA04658

Size:100 ul

Sample (30 ug of whole cell lysate)
A: 293T
B: A431
C: Raji
7.5% SDS PAGE
Glycyl-tRNA synthetase antibody
diluted at 1:1000

GARS antibody [N3C2], Internal detects GARS protein at cytosol on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma.
GARS antibody [N3C2], Internal dilution: 1:500.

GARS antibody [N3C2], Internal detects GARS protein at cytoplasm on human normal liver by immunohistochemical analysis.
Sample: Paraffin-embedded normal liver.
GARS antibody [N3C2], Internal dilution: 1:500.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: IHC-P, WB
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human GARS. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 0.37 mg/ml
• Preservative: 0.01% Thimerosal
• Application Info: Suggested starting dilutions are as follows: IHC-P: 1:100-1:1000, WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: GARS
• Gene Full Name: glycyl-tRNA synthetase
• Alias Symbols: GARS, HMN5, CMT2D, DSMAV, GlyRS, HMN5A, SMAD1, SMAJI
• NCBI Gene Id: 2617
• Protein Name: glycine--tRNA ligase
• Description of Target: This gene encodes glycyl-tRNA synthetase, one of the aminoacyl-tRNA synthetases that charge tRNAs with their cognate amino acids. The encoded enzyme is an (alpha)2 dimer which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. Two transcript variants encoding different isoforms have been found for this gene.
• Uniprot ID: P41250
• Protein Accession #: NP_001303701.1
• Nucleotide Accession #: NM_001316772.1