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GAPDH ELISA Kit (Human) : 96 Wells (OKEH01375)

96 Wells
$529.00
In Stock
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Predicted Species Reactivity:
Human
Reconstitution and Storage:
Store as indicated in product manual.
Gene Symbol:
GAPDH
Official Gene Full Name:
The protein encoded by this gene is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in
Alias Symbols:
CDABP0047, G3PD, GAPD, Glyceraldehyde-3-phosphate dehydrogenase, MGC88685, OK/SW-cl.12, Peptidyl-cysteine S-nitrosylase GAPDH
NCBI Gene Id:
2597
Protein Name:
Glyceraldehyde-3-phosphate dehydrogenase
Description of Target:
This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants.
Swissprot Id:
P04406
Protein Accession #:
NP_001243728.1
Nucleotide Accession #:
NM_001256799.2
Sample Type:
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity:
0.1 ng/mL
Kit Range:
0.156 - 10ng/mL
Kit Reproducibility:
Mean Intra-assay CV%: < 4.7% (n = 20)
Mean Inter-assay CV%: < 7.8% (n = 20)
Kit Duration:
~ 3 Hours
Kit Principle:
Aviva Systems Biology GAPDH ELISA Kit (Human) (OKEH01375) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for GAPDH has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for GAPDH is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample GAPDH captured in well.
Kit Component:
ComponentAmount
GAPDH Microplate96 Wells (12 x 8 Well strips)
GAPDH Lyophilized Standard2
100X Biotinylated GAPDH Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Kit Recovery:
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 92%
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express GAPDH.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express GAPDH.
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Specificity:
Natural and recombinant Human Glyceraldehyde-3-phosphate dehydrogenase
Assay Info:
Assay Methodology: Quantitative Sandwich ELISA
Molecular Weight:
36 kDa
Protein Size (# AA):
335

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