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- Phone: 858-552-6979
- Email: info@avivasysbio.com
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
| Datasheets/Manuals | Printable datasheet for anti-GAPDH (OAAJ03261) antibody |
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| Predicted Species Reactivity | Human, Mouse, Rat, Pig, Bovine, Goat, Monkey, Chicken |
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| Product Format | Liquid. PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol |
| Clonality | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Application | WB, IHC, IF |
| Additional Information | Subcellular Location: Cytoplasm > cytosol. Nucleus. Cytoplasm > perinuclear region. Membrane. Translocates to the nucleus following Snitrosylation and interaction with SIAH1, which contains a nuclear localization signal. Postnuclear and Perinuclear regions. Similarity: The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.Belongs to the glyceraldehyde-3-phosphate dehydrogenase family. |
| Reconstitution and Storage | Store at -20°C. Stable for 12 months from date of receipt. |
| Immunogen | A synthesized peptide derived from human GAPDH |
| Purification | The antiserum was purified by peptide affinity chromatography |
| Concentration | 1 mg/ml |
| Specificity | GAPDH antibody detects endogenous levels of total GAPDH |
| Application Info | WB: 1:3000~30000. For western blot, incubate membrane with diluted primary Ab in 5% w/v milk, 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking overnight IHC: 1:200 IF: 1:200 |
| Gene Symbol | GAPDH |
|---|---|
| Gene Full Name | glyceraldehyde-3-phosphate dehydrogenase |
| Alias Symbols | G3PD, GAPD, HEL-S-162eP |
| NCBI Gene Id | 2597 |
| Description of Target | Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) iswell known as one of the key enzymes involved in glycolysis.As well as functioning as a glycolytic enzyme in cytoplasm, recent evidence suggests that mammalian GAPDHis also involved in a great number of intracellular procesessuch as membrane fusion, microtubule bundling, phosphotransferaseactivity, nuclear RNA export, DNA replication, and DNA repair. |
| Uniprot ID | P04406 |
| Molecular Weight | 37 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "GAPDH Antibody (OAAJ03261)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Mouse, Rat, Pig, Bovine, Goat, Monkey, Chicken".
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How long will it take to receive "GAPDH Antibody (OAAJ03261)"?
This item is available "Domestic: within 2-3 week delivery | International: 2-3 weeks".
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What buffer format is "GAPDH Antibody (OAAJ03261)" provided in?
This item is provided in "Liquid. PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "GAPDH Antibody (OAAJ03261)"?
This target may also be called "G3PD, GAPD, HEL-S-162eP" in publications.
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What is the shipping cost for "GAPDH Antibody (OAAJ03261)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "GAPDH Antibody (OAAJ03261)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "GAPDH Antibody (OAAJ03261)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "37 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "GAPDH Antibody (OAAJ03261)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "GAPDH"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "GAPDH"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "GAPDH"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "GAPDH"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "GAPDH"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "GAPDH"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.
