Size:0.1ml
$230.00
In Stock
SKU
OAAD00231
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More Information
Predicted Species Reactivity Human
Product Format Ascitic fluid containing 0.03% sodium azide.
Clonality Monoclonal
Clone 1A10
Isotype IgG1
Host Mouse
Application WB, IHC, IF
Reconstitution and Storage Store at 4C, for long term storage, store at -20C.
Replacement Item This antibody may replace item sc-113612, HPA040067
Immunogen Purified recombinant fragment of human GAPDH expressed in E. coli.
Datasheets/Manuals Printable datasheet for GAPDH Antibody (OAAD00231) - OAAD00231
Application Info Western Blot: 1/500 - 1/2000.
Immunohistochemistry: 1/200 - 1/1000.
Immunofluorescence: 1/200 - 1/1000.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Publications

Kam, A. et al. Gallic acid protects against endothelial injury by restoring the depletion of DNA methyltransferase 1 and inhibiting proteasome activities. Int. J. Cardiol. 171, 231-42 (2014). WB, IHC, IF, ELISA, Human 24388544

Target Reference 1. Allen R.W. J. Biol. Chem. 1987.262:649-653.
2. Sumner CJ. Ann Neurol 2003.54:6 47-54.
Gene Symbol GAPDH
Alias Symbols G3PD, GAPD, MGC88685
NCBI Gene Id 2597
Protein Name Glyceraldehyde-3-phosphate dehydrogenase
Description of Target Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. It catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Besides its functioning as a glycolytic enzyme in cytoplasm, recent evidence suggest that mammalian GAPDH is also involved in a great number of intracellular proceses such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. During the last decade a lot of findings appeared concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death, age- related neuronal diseases, such as Alzheimer's and Huntington's disease.
Swissprot Id P04406
Molecular Weight 37kDa
Tissue Tool Find tissues and cell lines supported by DNA array analysis to express GAPDH.
RNA Seq Find tissues and cell lines supported by RNA-seq analysis to express GAPDH.
  1. What is the species homology for "GAPDH Antibody (OAAD00231)"?

    The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human".

  2. How long will it take to receive "GAPDH Antibody (OAAD00231)"?

    This item is available "Domestic: within 1 week delivery | International: 1 week".

  3. What buffer format is "GAPDH Antibody (OAAD00231)" provided in?

    This item is provided in "Ascitic fluid containing 0.03% sodium azide.".
    Additional format options may be available. For more information please contact info@avivasysbio.com.

  4. What are other names for "GAPDH Antibody (OAAD00231)"?

    This target may also be called "G3PD, GAPD, MGC88685" in publications.

  5. What is the shipping cost for "GAPDH Antibody (OAAD00231)"?

    The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.

  6. What is the guarantee for "GAPDH Antibody (OAAD00231)"?

    All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.

  7. Can I get bulk pricing for "GAPDH Antibody (OAAD00231)"?

    You can get bulk pricing for this item by going here.

  8. What is the molecular weight of the protein?

    The molecular weight reported by Uniprot for this item is "37kDa".
    Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.

  9. What protocols are available for "GAPDH Antibody (OAAD00231)"?

    We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data tab on the product page.

  10. What are positive controls for "GAPDH"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  11. What are negative controls for "GAPDH"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  12. What other proteins interact with "GAPDH"?

    This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.

  13. What biological processes are associated with "GAPDH"?

    This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.

  14. What cellular components are associated with "GAPDH"?

    This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.

  15. What protein functions are associated with "GAPDH"?

    This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.

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