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GAPDH Antibody (OAAD00231)

0.1ml
$230.00
In Stock
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Gene Symbol:
GAPDH
NCBI Gene Id:
2597
Protein Name:
Glyceraldehyde-3-phosphate dehydrogenase
Swissprot Id:
P04406
Alias Symbols:
G3PD, GAPD, MGC88685
Replacement Item:
This antibody may replace item sc-113612, HPA040067
Description of Target:
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. It catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Besides its functioning as a glycolytic enzyme in cytoplasm, recent evidence suggest that mammalian GAPDH is also involved in a great number of intracellular proceses such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. During the last decade a lot of findings appeared concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death, age- related neuronal diseases, such as Alzheimer's and Huntington's disease.
Molecular Weight:
37kDa
Host:
Mouse
Clonality:
Monoclonal
Application:
WB, IHC, IF, ELISA
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express GAPDH.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express GAPDH.
Immunogen:
Purified recombinant fragment of human GAPDH expressed in E. coli.
Predicted Species Reactivity:
Human
Product Format:
Ascitic fluid containing 0.03% sodium azide.
Reconstitution and Storage:
Store at 4C, for long term storage, store at -20C.
Datasheets/Manuals:
Printable datasheet for GAPDH Antibody (OAAD00231) - OAAD00231
Clone:
1A10
Isotype:
IgG1
Application Info:
Western Blot: 1/500 - 1/2000.
Immunohistochemistry: 1/200 - 1/1000.
Immunofluorescence: 1/200 - 1/1000.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Publications:

Kam, A. et al. Gallic acid protects against endothelial injury by restoring the depletion of DNA methyltransferase 1 and inhibiting proteasome activities. Int. J. Cardiol. 171, 231-42 (2014). WB, IHC, IF, ELISA, Human 24388544

Target Reference:
1. Allen R.W. J. Biol. Chem. 1987.262:649-653.
2. Sumner CJ. Ann Neurol 2003.54:6 47-54.

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