- Predicted Species Reactivity:
- Product Format:
- Ascitic fluid containing 0.03% sodium azide.
- WB, IHC, IF
- Reconstitution and Storage:
- Store at 4C, for long term storage, store at -20C.
- Gene Symbol:
- NCBI Gene Id:
- Protein Name:
- Glyceraldehyde-3-phosphate dehydrogenase
- Swissprot Id:
- Alias Symbols:
- G3PD, GAPD, MGC88685
- Replacement Item:
- This antibody may replace item sc-113612, HPA040067
- Description of Target:
- Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. It catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Besides its functioning as a glycolytic enzyme in cytoplasm, recent evidence suggest that mammalian GAPDH is also involved in a great number of intracellular proceses such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. During the last decade a lot of findings appeared concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death, age- related neuronal diseases, such as Alzheimer's and Huntington's disease.
- Molecular Weight:
- Tissue Tool:
- Find tissues and cell lines supported by DNA array analysis to express GAPDH.
- RNA Seq:
- Find tissues and cell lines supported by RNA-seq analysis to express GAPDH.
- Purified recombinant fragment of human GAPDH expressed in E. coli.
- Printable datasheet for GAPDH Antibody (OAAD00231) - OAAD00231
- Application Info:
- Western Blot: 1/500 - 1/2000.
Immunohistochemistry: 1/200 - 1/1000.
Immunofluorescence: 1/200 - 1/1000.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Kam, A. et al. Gallic acid protects against endothelial injury by restoring the depletion of DNA methyltransferase 1 and inhibiting proteasome activities. Int. J. Cardiol. 171, 231-42 (2014). WB, IHC, IF, ELISA, Human 24388544
- Target Reference:
- 1. Allen R.W. J. Biol. Chem. 1987.262:649-653.
2. Sumner CJ. Ann Neurol 2003.54:6 47-54.
- Tips Information: