GAPDH Antibody (OAAD00231)

Catalog No: OAAD00231

Size:0.1ml

Western blot analysis using GAPDH mouse mAb against Hela (1), A549 (2), A431 (3), MCF-7 (4), K562 (5), Jurkat (6), HL60 (7), SKN-SH (8) and SKBR-3 (9) cell lysate.

Immunohistochemical analysis of paraffin-embedded human breast carcinoma (left) and kidney carcinoma (right), showing cytoplasmic localization using GAPDH mouse mAb with DAB staining.

Confocal immunofluorescence analysis of methanol-fixed HepG2 (left) and Hela (right) cells using GAPDH mouse mAb (green), showing cytoplasmic localization. Blue: DRAQ5 fluorescent DNA dye.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid containing 0.03% sodium azide.
• Clonality: Monoclonal
• Clone: 1A10
• Isotype: IgG1
• Host: Mouse
• Application: WB, IHC, IF
• Reconstitution and Storage: Store at 4°C, for long term storage, store at -20°C.
• Immunogen: Purified recombinant fragment of human GAPDH expressed in E. coli.
• Concentration: 0.5 mg/ml
• Application Info: WB: 1/500 - 1/2000
  IHC: 1/200 - 1/1000
  IF: 1/200 - 1/1000
  ELISA: Propose dilution 1/10000
• Publications:

  Expression of Kruppel-like factor 8 and Ki67 in lung adenocarcinoma and prognosis. Exp Ther Med. 14, 1351-1356 (2017). 28810596

  Kam, A. et al. Gallic acid protects against endothelial injury by restoring the depletion of DNA methyltransferase 1 and inhibiting proteasome activities. Int. J. Cardiol. 171, 231-42 (2014). 24388544

• Reference: 1. Allen R.W. J. Biol. Chem. 1987.262:649-653.
  2. Sumner CJ. Ann Neurol 2003.54:6 47-54.
• Description:
  View product citations for GAPDH Antibody (OAAD00231) on CiteAb

Target Info

• Gene Symbol: GAPDH
• Alias Symbols: G3PD, GAPD, HEL-S-162eP
• NCBI Gene Id: 2597
• Protein Name: Glyceraldehyde-3-phosphate dehydrogenase
• Description of Target: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. It catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Besides its functioning as a glycolytic enzyme in cytoplasm, recent evidence suggest that mammalian GAPDH is also involved in a great number of intracellular proceses such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. During the last decade a lot of findings appeared concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death, age- related neuronal diseases, such as Alzheimer's and Huntington's disease.
• Uniprot ID: P04406
• Molecular Weight: 37 kDa