GAPDH Antibody - N-terminal region (ARP40175_P050)

Catalog No: ARP40175_P050

Host: Rabbit
Target Name: GAPDH
Sample Type: 293T
Lane A: Primary Antibody
Lane B: Primary Antibody + Blocking Peptide
Primary Antibody Concentration: 1ug/ml
Peptide Concentration: 5ug/ml
Lysate Quantity: 25ug/lane/lane
Gel Concentration: 0.12

Host: Rabbit
Target Name: GAPDH
Sample Type: Hela Whole Cell lysates
Antibody Dilution: 1.0ug/mlGAPDH is strongly supported by BioGPS gene expression data to be expressed in Human HeLa cells

WB Suggested Anti-GAPDH Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: Human brain

Lanes:
1. 50 ug NIH3T3 cytosolic extract 2. 50 ug NIH3T3 cytosolic extract 3. 50 ug NIH3T3 cytosolic extract 4. 50 ug NIH3T3 cytosolic extract
Primary Antibody Dilution:
1:1000
Secondary Antibody:
Anti-Rabbit HRP
Secondary Antibody Dilution:
1:30000
Gene Name:
GAPDH
Submitted by:
Dr. Arimdam Basu, Dept. of Animal Biology, University of Pennsylvania

WB Suggested Anti-GAPDH antibody Titration: 1 ug/mL
Sample Type: Human Raji

Rabbit Anti-GAPDH Antibody
Catalog Number: ARP40175_P050
Formalin Fixed Paraffin Embedded Tissue: Human Heart Muscle Tissue
Observed Staining: Cytoplasm
Primary Antibody Concentration: 1:600
Other Working Concentrations: N/A
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec

Rabbit Anti-GAPDH Antibody
Catalog Number: ARP40175_P050
Formalin Fixed Paraffin Embedded Tissue: Human Liver Tissue
Observed Staining: Cytoplasm in hepatocytes
Primary Antibody Concentration: 1:100
Other Working Concentrations: N/A
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec

25 ug of the indicated Human whole cell or tissue extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment. An isoform containing the peptide sequence is present at 84 kDa.

Product Info

• Publications:

  Fu, Y. et al. ABCA12 Regulates ABCA1-Dependent Cholesterol Efflux from Macrophages and the Development of Atherosclerosis. Cell Metab. 18, 225-38 (2013). 23931754

  Kalinec, G. M. et al. Acetaminophen and NAPQI are toxic to auditory cells via oxidative and endoplasmic reticulum stress-dependent pathways. Hear. Res. 313, 26-37 (2014). 24793116

  More...

• Tested Species Reactivity: Human, Mouse
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Goat, Guinea Pig, Horse, Rabbit, Sheep
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB, IHC
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human GAPDH
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 93%; Dog: 100%; Goat: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 93%; Rabbit: 100%; Rat: 100%; Sheep: 100%
• Peptide Sequence: Synthetic peptide located within the following region: IDLNYMVYMFQYDSTHGKFHGTVKAENGKLVINGNPITIFQERDPSKIKW
• Concentration: 0.5 mg/ml
• Blocking Peptide: For anti-GAPDH (ARP40175_P050) antibody is Catalog # AAP40175 (Previous Catalog # AAPP22021)
• Sample Type Confirmation:

  GAPDH is strongly supported by BioGPS gene expression data to be expressed in HeLa

• Enhanced Validation:

  Relative Expression (Western Blot)

Target Info

• Reference: Rinne,T., (2008) Hum. Mol. Genet. 17 (13), 1968-1977
• Gene Symbol: GAPDH
• Gene Full Name: Glyceraldehyde-3-phosphate dehydrogenase
• Alias Symbols: G3PD, GAPD, HEL-S-162eP
• NCBI Gene Id: 2597
• Protein Name: Glyceraldehyde-3-phosphate dehydrogenase RuleBase RU003389
• Description of Target: GAPDH catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome.Glyceraldehyde-3-phosphate dehydrogenase catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. A GAPD pseudogene has been mapped to Xp21-p11 and 15 GAPD-like loci have been identified. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
• Uniprot ID: Q2TSD0
• Protein Accession #: NP_002037
• Nucleotide Accession #: NM_002046
• Protein Size (# AA): 335
• Molecular Weight: 36 kDa
• Protein Interactions: FUS; HUWE1; UBC; SUMO2; SUMO3; STAU1; MDM2; GAPDH; ASB15; ASB16; RPA3; RPA2; RPA1; EED; SNCA; rev; HSPA9; HSPA1L; GAS7; TUBA1A; TUFM; RAP1GDS1; PPP5C; PPIB; YWHAG; TUBB; TUBA1C; PRPF40A; UBQLN1; DCPS; MAT2B; ACOT7; METAP2; FERMT2; GBP1; EEF1A1; TYMP; DFFA