GAPDH Antibody - FITC Conjugated (OACA00044)

Catalog No: OACA00044

Size:100ug

All lanes : Glyceraldehyde-3-phosphate dehydrogenase antibody at at 2 ug/ml
Lane 1 :EC109 whole cell lysate
Lane 2 :293T whole cell lysate
Secondary Goat polyclonal to Rabbit IgG at 1/15000 dilution
Predicted band size : 37kDa
Observed band size: 37kDa

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Conjugation: FITC
• Application: WB
• Reconstitution and Storage: -20°C or -80°C
• Immunogen: Recombinant Human Glyceraldehyde-3-phosphate dehydrogenase protein (3-335AA)
• Purification: Protein G Purified
• Purity: Caprylic Acid Ammonium Sulfate Precipitation purified
• Reference: [1] Nucleotide sequence and gene-polypeptide relationships of the glpABC operon encoding the anaerobic sn-glycerol-3-phosphate dehydrogenase of Escherichia coli K-12.'Cole S.T., Eiglmeier K., Ahmed S., Honore N., Elmes L., Anderson W.F., Weiner J.H.J. Bacteriol. 170:2448-2456(1988)
  [2] Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features.'Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T.Horiuchi T.DNA Res. 4:91-113(1997)
  [3] The complete genome sequence of Escherichia coli K-12.'Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.Science 277:1453-1474(1997)
• Storage Buffer: Preservative: 0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Target Info

• Gene Symbol: GAPDH
• Gene Full Name: glyceraldehyde-3-phosphate dehydrogenase
• Alias Symbols: aging-associated gene 9 protein;epididymis secretory sperm binding protein Li 162eP;G3PD;GAPD;glyceraldehyde-3-phosphate dehydrogenase;HEL-S-162eP;OCAS, p38 component;Oct1 coactivator in S phase, 38 Kd component;peptidyl-cysteine S-nitrosylase GAPDH.
• NCBI Gene Id: 2597
• Protein Name: Glyceraldehyde-3-phosphate dehydrogenase
• Description of Target: Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation.
• Uniprot ID: P04406