GAPDH Antibody - C-terminal region (OAEB02389)

Catalog No: OAEB02389 (Formerly GWB-3A8568)

Size:100UG

OAEB02389 staining (0.01ug/ml) of 293 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Product Info

• Predicted Species Reactivity: Dog|Human|Mouse|Porcine|Rat
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: WB
• Additional Information: Reactivity Info: Tested: Human, Mouse; Expected: Human, Mouse, Dog
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: GAPDH
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Horse: 90%; Human: 100%; Pig: 90%; Sheep: 90%
• Peptide Sequence: C-HQVVSSDFNSDT
• Concentration: 0.5 mg/ml
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Application Info: Peptide ELISA: antibody detection limit dilution 1:2000.
  Western Blot: Approx 35kDa band observed in lysates of Human Tonsil, Mouse Spleen, and cell lines HeLa, A431 and 293 (calculated MW of 36.1kDa according to NP_002037). Recommended concentration: 0.01-0.03ug/ml.
• Reference: * Carr AJ, Vugler A, Lawrence J, Chen LL, Ahmado A, Chen FK, Semo M, Gias C, da Cruz L, Moore HD, Walsh J, Coffey PJ.; Molecular characterization and functional analysis of phagocytosis by human embryonic stem cell-derived RPE cells using a novel human retinal assay.; Mol Vis. 2009;15:283-95. Epub 2009 Feb 6.; Pubmed 19204785
  * Kiepe D, Van Der Pas A, Ciarmatori S, Standker L, Schutt B, Hoeflich A, Hugel U, Oh J, Tonshoff B.; Defined carboxy-terminal fragments of insulin-like growth factor (IGF) binding protein 2 exert similar mitogenic activity on cultured rat growth plate chondrocytes as IGF-I.; Endocrinology. 2008 Oct;149(10):4901-11. Epub 2008 Jun 12.; Pubmed 18556354
  * Henson MA, Roberts AC, Salimi K, Vadlamudi S, Hamer RM, Gilmore JH, Jarskog LF, Philpot BD.; Developmental regulation of the NMDA receptor subunits, NR3A and NR1, in human prefrontal cortex.; Cereb Cortex. 2008 Nov;18(11):2560-73.; Pubmed 18296432
  * Gerstner JR, Bremer QZ, Vander Heyden WM, Lavaute TM, Yin JC, Landry CF.; Brain fatty acid binding protein (Fabp7) is diurnally regulated in astrocytes and hippocampal granule cell precursors in adult rodent brain.; PLoS One. 2008 Feb 20;3(2):e1631.; Pubmed 18286188
  * Nguyen M, Pace AJ, Koller BH.; Mice lacking NKCC1 are protected from development of bacteremia and hypothermic sepsis secondary to bacterial pneumonia.; J Exp Med. 2007 Jun 11;204(6):1383-93.; Pubmed 17517966
  * Burke JR, Enghild JJ, Martin ME, Jou YS, Myers RM, Roses AD, Vance JM,
  Strittmatter WJ.; Huntingtin and DRPLA proteins selectively interact with the enzyme GAPDH.; Nat Med. 1996 Mar;2(3):347-50.; Pubmed 8612237

Target Info

• Gene Symbol: GAPDH
• Gene Full Name: glyceraldehyde-3-phosphate dehydrogenase
• Alias Symbols: aging-associated gene 9 protein;epididymis secretory sperm binding protein Li 162eP;G3PD;GAPD;glyceraldehyde-3-phosphate dehydrogenase;HEL-S-162eP;OCAS, p38 component;Oct1 coactivator in S phase, 38 Kd component;peptidyl-cysteine S-nitrosylase GAPDH.
• NCBI Gene Id: 2597
• Protein Name: Glyceraldehyde-3-phosphate dehydrogenase
• Uniprot ID: P04406
• Protein Accession #: NP_002037.2
• Molecular Weight: 36.1