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GAPDH (2A8) mAb (OAAH00016)

100 ul
$185.00
In Stock
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Predicted Species Reactivity:
Human, Rat, Mouse, Monkey, Dog, Chinese hamster
Clonality:
Monoclonal
Isotype:
Mouse IgG1
Host:
Mouse
Application:
WB
Reconstitution and Storage:
Store the product at -20C. Stable for one year from the date of shipment.
Gene Symbol:
GAPDH
NCBI Gene Id:
2597
Protein Name:
Glyceraldehyde-3-phosphate dehydrogenase
Swissprot Id:
P04406
Alias Symbols:
G3PD, GAPD, HEL-S-162eP,
Replacement Item:
This antibody may replace item sc-113612 from Santa Cruz Biotechnology.
Description of Target:
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. Though differentially expressed from tissue to tissue, GAPDH is thought to be a constitutively expressed housekeeping protein. For this reason, GAPDH mRNA and protein levels are often measured as controls in experiments quantifying specific changes in expression of other targets. Recent work has elucidated roles for GAPDH in apoptosis, gene expression and nuclear transport. GAPDH may also play a role in neurodegenerative pathologies such as Huntington and Alzheimer's diseases.
Molecular Weight:
37 kDa
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express GAPDH.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express GAPDH.
Immunogen:
This Aviva monoclonal antibody is produced by immunizing animals with a synthetic peptide (KLH-coupled) of human GAPDH.
Datasheets/Manuals:
Printable datasheet for GAPDH (2A8) mAb (OAAH00016) OAAH00016
Specificity:
GAPDH (2A8) Mouse mAb detects endogenous levels of total GAPDH protein.
Source:
Mouse mAb
Application Info:
WB: 1: 2000-1:5000
Target Reference:
1. Barber, R.D. et al. 2005. GAPDH as a housekeeping gene: analysis of
GAPDH mRNA expression in a panel of 72 human tissues. Physiol.
Genomics 21, 389-95.
2. Hara, M.R. and Snyder, S.H. 2006. Nitric oxide-GAPDH-Siah: a novel cell
death cascade. Cell Mol. Neurobiol. 26, 527-38.
3. Zheng, L. et al. 2003. S phase activation of the histone H2B promoter by
OCA-S, a coactivator complex that contains GAPDH as a key component.
Cell 114, 255-66.
4. Bae, B.I. et al. 2006. Mutant huntingtin: nuclear translocation and
cytotoxicity mediated by GAPDH. Proc. Natl. Acad. Sci. USA 103, 3405-9.
5. Wang, Q. et al. 2005. Proteomic analysis of neurofibrillary tangles in
Alzheimer disease identifies GAPDH as a detergent-insoluble paired
helical filament tau binding protein. FASEB J. 19, 869-71.

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