GANAB Antibody (OAGA00173)

Catalog No: OAGA00173

Size:100 ul

Sample(30 ug whole cell lysate)
A: 293T
B: A431
C: H1299
D: HeLa S3
E: Hep G2
F: MOLT4
G: Raji
7.5% SDS PAGE
OAGA00173 diluted at 1:1000

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
OAGA00173 diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using alpha Glucosidase II(OAGA00173) antibody at 1:500 dilution.

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using alpha Glucosidase II(OAGA00173) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with(Red) at 1:2000.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC-FFPE, WB
  Positive Controls: 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji, mouse brain
  Cellular Localization: Endoplasmic reticulum (By similarity), Golgi apparatus (By similarity), Melanosome
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human alpha Glucosidase II. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 1 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: GANAB
• Gene Full Name: Neutral alpha-glucosidase AB
• Alias Symbols: G2AN, GIIA, PKD3, GLUII
• NCBI Gene Id: 23193
• Protein Name: Neutral alpha-glucosidase AB
• Uniprot ID: Q14697
• Protein Accession #: NP_938148.1
• Molecular Weight: 109 kDa