Catalog No: OKCA00681
Size:96 Wells
Price: $750.00
SKU
OKCA00681
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetClick Hereto download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3.5 Hours
ELISA Kit Linearity
DilutionMean n=4 (%)Range (%)
Neat9791-100
1:210399-107
1:49487-97
1:89891-102
ELISA Kit PrincipleAviva Systems Biology GALT ELISA Kit (Human) (OKCA00681) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for GALT has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for GALT is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample GALT captured in the well.
ELISA Kit Range31.25 pg/mL-2,000 pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)9084-94
EDTA Plasma (n=4)9488-97
ELISA Kit ReproducibilityIntra-assay Precision: 3 samples with known low, middle and high level GALT were tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level GALT were tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <8%
Inter-Assay CV: <10%
ELISA Kit Component
ComponentAmount
GALT Microplate96 Wells (12 x 8 Well strips)
GALT Lyophilized Standard2
100X Biotinylated GALT Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 50 mL
Detector Antibody Diluent1 x 15 mL
Conjugate Diluent1 x 15 mL
25X Wash Buffer1 x 20 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates
Sensitivity7.81 pg/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolGALT
Gene Full NameGalactose-1-phosphate uridylyltransferase
Alias SymbolsGal-1-P uridylyltransferase, Galactose-1-phosphate uridylyltransferase, UDP-glucose--hexose-1-phosphate uridylyltransferase
NCBI Gene Id2592
Protein NameGalactose-1-phosphate uridylyltransferase
Description of TargetGalactose-1-phosphate uridyl transferase (GALT) catalyzes the second step of the Leloir pathway of galactose metabolism, namely the conversion of UDP-glucose + galactose-1-phosphate to glucose-1-phosphate + UDP-galactose. The absence of this enzyme results in classic galactosemia in humans and can be fatal in the newborn period if lactose is not removed from the diet. The pathophysiology of galactosemia has not been clearly defined. Two transcript variants encoding different isoforms have been found for this gene.
Uniprot IDP07902
Protein Accession #NP_000146.2
Nucleotide Accession #NM_000155.3
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