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- $55: Antibody & Protein in US
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Datasheets/Manuals | Printable datasheet for anti-GALR2 antibody - OAGA00042 |
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Predicted Species Reactivity | Human |
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Product Format | Liquid |
Clonality | Polyclonal |
Isotype | IgG |
Host | Rabbit |
Application | IHC-FFPE, WB |
Additional Information | Background: Galanin is an important neuromodulator present in the brain, gastrointestinal system, and hypothalamopituitary axis. It is a 30-amino acid non-C-terminally amidated peptide that potently stimulates growth hormone secretion, inhibits cardiac vagal slowing of heart rate, abolishes sinus arrhythmia, and inhibits postprandial gastrointestinal motility. The actions of galanin are mediated through interaction with specific membrane receptors that are members of the 7-transmembrane family of G protein-coupled receptors. GALR2 interacts with the N-terminal residues of the galanin peptide. The primary signaling mechanism for GALR2 is through the phospholipase C/protein kinase C pathway (via Gq), in contrast to GALR1, which communicates its intracellular signal by inhibition of adenylyl cyclase through Gi. However, it has been demonstrated that GALR2 couples efficiently to both the Gq and Gi proteins to simultaneously activate 2 independent signal transduction pathways. [provided by RefSeq] |
:: | Positive Controls: 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji |
:: | Cellular Localization: Cell membrane; Multi-pass membrane protein |
Reconstitution and Storage | Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles. |
Immunogen | Synthetic peptide corresponding to a region within amino acids 212 and 307 of Galanin Receptor 2 (Uniprot ID#O43603) |
Purification | Purified by antigen-affinity chromatography. |
Concentration | 1 mg/ml |
Application Info | Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
Gene Symbol | GALR2 |
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Gene Full Name | Galanin receptor type 2 |
Alias Symbols | GAL2-R, GALNR2, GALR-2 |
NCBI Gene Id | 8811 |
Protein Name | Galanin receptor type 2 |
Uniprot ID | O43603 |
Protein Accession # | NP_003848.1 |
Molecular Weight | 42 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "GALR2 Antibody (OAGA00042)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human".
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How long will it take to receive "GALR2 Antibody (OAGA00042)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "GALR2 Antibody (OAGA00042)" provided in?
This item is provided in "Liquid".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "GALR2 Antibody (OAGA00042)"?
This target may also be called "GAL2-R, GALNR2, GALR-2" in publications.
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What is the shipping cost for "GALR2 Antibody (OAGA00042)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "GALR2 Antibody (OAGA00042)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "GALR2 Antibody (OAGA00042)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "42 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "GALR2 Antibody (OAGA00042)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "GALR2"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "GALR2"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "GALR2"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "GALR2"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "GALR2"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "GALR2"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.