Catalog No: OKEH04440
Price: $525.00
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology Gal ELISA Kit (Rat) (OKEH04440) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Gal has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for Gal is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Gal captured in the well.
ELISA Kit Range0.312-20ng/mL
ELISA Kit Component
Anti-Gal Microplate96 Wells (12 x 8 Well Strips)
Gal Lyophilized Standard2 x 2 ng
Sample Diluent1 x 20 mL
Gal Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity0.158 ng/mL
SpecificityNatural and recombinant Rat Galanin peptides
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolGal
Gene Full Namegalanin and GMAP prepropeptide
Alias Symbolsgalanin peptides;galanin prepropeptide;galanin/GMAP prepropeptide;Galn.
NCBI Gene Id29141
Protein NameP10683
Description of TargetGalanin peptides
Uniprot ID
Protein Accession #NP_150240.1
Nucleotide Accession #NM_033237.1
Protein Size (# AA)124
Molecular Weight13 KDa
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