Catalog No: OKEH00751
Price: $600.00
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GAL ELISA Kit (Mouse) : 96 Wells (OKEH00751)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology GAL ELISA Kit (Mouse) (OKEH00751) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human GAL antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated GAL and incubated. The GAL found in the sample or standards competes with the biotinylated GAL for limited binding sites on the immobilized anti-human GAL antibody. Excess unbound biotinylated GAL and sample or standard GAL is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated GAL captured in the well and inversely proportional to the amount of GAL which was contained in the sample or standard.
ELISA Kit Range31.2-2000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 98%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.9% (n = 20)
Mean Inter-assay CV%: < 8.6% (n = 20)
ELISA Kit Component
Anti-GAL Microplate96 Wells (12 x 8 Well strips)
GAL Lyophilized Standard2
Sample Diluent1 x 20 mL
100X GAL-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity15.6 pg/mL
SpecificityNatural and recombinant Mouse Galanin peptides
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Gene SymbolGal
Gene Full Namegalanin and GMAP prepropeptide
Alias SymbolsG;galanin;galanin peptides;Galn.
NCBI Gene Id14419
Protein NameP47212
Description of TargetGalanin peptides
Uniprot ID
Protein Accession #NP_034383.1
Nucleotide Accession #NM_010253.3
Protein Size (# AA)124
Molecular Weight13 kDa
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