GAL Antibody (OAEB01591)

Catalog No: OAEB01591 (Formerly GWB-EV0706)

Size:100UG

OAEB01591 (1ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

OAEB01591 (0.3ug/ml) staining of PFA-perfused cryosection of Human Hypothalamus. Antigen retrieval with citrate buffer pH 6 at 80C for 30min, Cy3-staining. Data obtained by Prof. Erik Hrabovszky, Inst, Exp, Med., Budapest, Hungary.

Product Info

• Predicted Species Reactivity: Bovine|Human|Mouse|Rat
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: P-WB, IHC
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: GAL
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human, Mouse, Rat, Cow
• Peptide Sequence: C-HRSFSDKNGLTSK
• Concentration: 0.5 mg/ml
• Application Info: ELISA: antibody detection limit dilution 1:32000
  WB: Approx 17kDa band observed in Human Brain (Cerebellum) and in Mouse and Rat Brain lysates (calculated MW of 13.3 kDa according to Human NP_057057.2 and of 13.5 kDa according to Mouse NP_034383.1 and Rat NP_150240.1). Recommended concentration: 1-3 ug/ml. Primary incubation was 1 hour.
  IHC: Frozen section of Human Hypothalamus shows staining of galaninergic axons in the human periventricular nucleus. Recommended concentration: 0.2-0.5 ug/ml

Target Info

• Gene Symbol: Gal|GAL
• Gene Full Name: galanin and GMAP prepropeptide
• Alias Symbols: ETL8;G;galanin;galanin peptides;galanin prepropeptide;galanin/GMAP prepropeptide;galanin-message-associated peptide;galanin-related peptide;GAL-GMAP;GALN;GLNN;GMAP.
• NCBI Gene Id: 14419|29141|51083
• Protein Name: Galanin peptides
• Uniprot ID: P22466
• Protein Accession #: NP_057057.2
• Molecular Weight: 13.3