GABPA ELISA Kit (Human) (OKCD00513)

Catalog No: OKCD00513 (Formerly GWB-KBBRH6)

Size:96WELLS

Human GABPA ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to GA Binding Protein Transcription Factor Alpha (GABPa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to GA Binding Protein Transcription Factor Alpha (GABPa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GA Binding Protein Transcription Factor Alpha (GABPa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of GA Binding Protein Transcription Factor Alpha (GABPa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level GA Binding Protein Transcription Factor Alpha (GABPa) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level GA Binding Protein Transcription Factor Alpha (GABPa) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-GABPA Microplate: 96 Wells (12 x 8 Well Strips)
  • GABPA Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated GABPA Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates and other biological fluids.
• Sensitivity: < 0.057 ng/mL.
• Specificity: This assay has high sensitivity and excellent specificity for detection of GA Binding Protein Transcription Factor Alpha (GABPa). No significant cross-reactivity or interference between GA Binding Protein Transcription Factor Alpha (GABPa) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: GABPA
• Gene Full Name: GA binding protein transcription factor subunit alpha
• Alias Symbols: E4TF1-60;E4TF1A;GA binding protein transcription factor alpha subunit 60kDa;GA-binding protein alpha chain;GABP subunit alpha;human nuclear respiratory factor-2 subunit alpha;NFT2;NRF2;NRF2A;nuclear respiratory factor 2 alpha subunit;nuclear respiratory factor 2 subunit alpha;RCH04A07;transcription factor E4TF1-60.
• NCBI Gene Id: 2551
• Protein Name: GA-binding protein alpha chain
• Description of Target: Transcription factor capable of interacting with purine rich repeats (GA repeats). Necessary for the expression of the Adenovirus E4 gene.
• Uniprot ID: Q06546
• Protein Accession #: NP_001184226
• Nucleotide Accession #: NM_001197297.1
• Protein Size (# AA): 454