GABARAPL2 ELISA Kit (Human) (OKCD00560)

Catalog No: OKCD00560 (Formerly GWB-KBBNT7)

Size:96WELLS

Human GABARAPL2 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to GABA-A Receptor Associated Protein Like Protein 2 (GABARAPL2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to GABA-A Receptor Associated Protein Like Protein 2 (GABARAPL2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GABA-A Receptor Associated Protein Like Protein 2 (GABARAPL2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of GABA-A Receptor Associated Protein Like Protein 2 (GABARAPL2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level GABA-A Receptor Associated Protein Like Protein 2 (GABARAPL2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level GABA-A Receptor Associated Protein Like Protein 2 (GABARAPL2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-GABARAPL2 Microplate: 96 Wells (12 x 8 Well Strips)
  • GABARAPL2 Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated GABARAPL2 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids.
• Sensitivity: < 0.061 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of GABA-A Receptor Associated Protein Like Protein 2 (GABARAPL2). No significant cross-reactivity or interference between GABA-A Receptor Associated Protein Like Protein 2 (GABARAPL2) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: GABARAPL2
• Gene Full Name: GABA type A receptor associated protein like 2
• Alias Symbols: ATG8;ATG8C;GABA(A) receptor-associated protein-like 2;gamma-aminobutyric acid receptor-associated protein-like 2;ganglioside expression factor 2;GATE16;GATE-16;GEF2;GEF-2;general protein transport factor p16;golgi-associated ATPase enhancer of 16 kDa;MAP1 light chain 3 related protein;MAP1 light chain 3-related protein.
• NCBI Gene Id: 11345
• Protein Name: Gamma-aminobutyric acid receptor-associated protein-like 2
• Description of Target: Ubiquitin-like modifier involved in intra-Golgi traffic. Modulates intra-Golgi transport through coupling between NSF activity and SNAREs activation. It first stimulates the ATPase activity of NSF which in turn stimulates the association with GOSR1 (By similarity). Involved in autophagy. Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation.
• Uniprot ID: P60520
• Protein Accession #: NP_009216
• Nucleotide Accession #: NM_007285.6
• Protein Size (# AA): 117