Size:96 Wells
$619.00
In Stock
SKU
OKEH02564
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GABA ELISA Kit (OKEH02564)

Catalog#: OKEH02564
Domestic: within 1 week delivery International: 1 week
More Information
Predicted Species ReactivityAll
ApplicationELISA
Kit Detection MethodColorimetric, OD 450 nm
Kit Duration~ 3 Hours
Kit PrincipleAviva Systems Biology GABA ELISA Kit (OKEH02564) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-GABA antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated GABA and incubated. The GABA found in the sample or standards competes with the biotinylated GABA for limited binding sites on the immobilized anti-GABA antibody. Excess unbound biotinylated GABA and sample or standard GABA is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated GABA captured in the well and inversely proportional to the amount of GABA which was contained in the sample or standard.
Kit Range1.56 - 100ng/mL
Kit RecoveryMean recovery when spiking into Serum and Plasma = 89%
Kit ReproducibilityMean Intra-assay CV%: < 5.1% (n = 20)
Mean Inter-assay CV%: < 6.9% (n = 20)
Kit Component
ComponentAmount
Anti-GABA Microplate96 Wells (12 x 8 Well strips)
GABA Lyophilized Standard2 x 100 ng
Sample Diluent1 x 20 mL
100X GABA-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationFunction: g-Aminobutyric acid is the chief inhibitory neurotransmitter in the mammalian central nervous system. It plays a role in regulating neuronal excitability throughout the nervous system. In humans, GABA is also directly responsible for the regulation of muscle tone. Although chemically it is an amino acid, GABA is rarely referred to as such in the scientific or medical communities, because the term "amino acid," used without a qualifier, conventionally refers to the alpha amino acids, which GABA is not, nor is it ever incorporated into a protein. In spastic diplegia in humans, GABA absorption becomes impaired by nerves damaged from the condition's upper motor neuron lesion, which leads to hypertonia of the muscles signaled by those nerves that can no longer absorb GABA.
::Pubchem: 119
::Chemical formula: C4H9NO2
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, tissue homogenates, cell culture supernatants and other biological fluids
Sensitivity0.45 ng/mL
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
SpecificityNatural and recombinant General Gamma-Aminobutyric acid
Publications

Alejandra Romo-Araiza, Gabriela Gutiérrez-Salmeán, Emilio J. Galván, Melissa Hernández-Frausto, Gabriel Herrera-López, Hector Romo-Parra, Valentina García-Contreras, Ana María Fernández-Presas, Ricardo Jasso-Chávez, Cesar V. Borlongan, & Antonio Ibarra1. Probiotics and Prebiotics as a Therapeutic Strategy to Improve Memory in a Model of Middle-Aged Rats. Front Aging Neurosci. 10, 416 (2018). ELISA, PMC6305305

Assay InfoAssay Methodology: Quantitative Competitive ELISA
Gene SymbolGABA
Official Gene Full NameGamma-Aminobutyric acid
Alias Symbols4-aminobutyric acid, 4-Aminobutanoic acid, GABA, Gamma-aminobutyric acid, Piperidic acid
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