|Predicted Species Reactivity
|Kit Detection Method
||Colorimetric, OD 450 nm
||~ 3 Hours
||Aviva Systems Biology GABA ELISA Kit (OKEH02564) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-GABA antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated GABA and incubated. The GABA found in the sample or standards competes with the biotinylated GABA for limited binding sites on the immobilized anti-GABA antibody. Excess unbound biotinylated GABA and sample or standard GABA is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated GABA captured in the well and inversely proportional to the amount of GABA which was contained in the sample or standard.
||1.56 - 100ng/mL
||Mean recovery when spiking into Serum and Plasma = 89%
||Mean Intra-assay CV%: < 5.1% (n = 20)
Mean Inter-assay CV%: < 6.9% (n = 20)
|Anti-GABA Microplate||96 Wells (12 x 8 Well strips)|
|GABA Lyophilized Standard||2 x 100 ng|
|Sample Diluent||1 x 20 mL|
|100X GABA-Biotin Complex||1 x 60 uL|
|100X Avidin-HRP Conjugate||1 x 120 uL|
|Biotin Complex Diluent||1 x 12 mL|
|Conjugate Diluent||1 x 12 mL|
|25X Wash Buffer||1 x 30 mL|
|TMB Substrate||1 x 10 mL|
|Stop Solution||1 x 10 mL|
||Function: g-Aminobutyric acid is the chief inhibitory neurotransmitter in the mammalian central nervous system. It plays a role in regulating neuronal excitability throughout the nervous system. In humans, GABA is also directly responsible for the regulation of muscle tone. Although chemically it is an amino acid, GABA is rarely referred to as such in the scientific or medical communities, because the term "amino acid," used without a qualifier, conventionally refers to the alpha amino acids, which GABA is not, nor is it ever incorporated into a protein. In spastic diplegia in humans, GABA absorption becomes impaired by nerves damaged from the condition's upper motor neuron lesion, which leads to hypertonia of the muscles signaled by those nerves that can no longer absorb GABA.
||Chemical formula: C4H9NO2
|Reconstitution and Storage
||Store as indicated in product manual.
||Serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids
||Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
||Printable COA datasheet for OKEH02564
||Natural and recombinant General Gamma-Aminobutyric acid
Alejandra Romo-Araiza, Gabriela GutiÃƒÂ©rrez-SalmeÃƒÂ¡n, Emilio J. GalvÃƒÂ¡n, Melissa HernÃƒÂ¡ndez-Frausto, Gabriel Herrera-LÃƒÂ³pez, Hector Romo-Parra, Valentina GarcÃƒÂa-Contreras, Ana MarÃƒÂa FernÃƒÂ¡ndez-Presas, Ricardo Jasso-ChÃƒÂ¡vez, Cesar V. Borlongan, & Antonio Ibarra1. Probiotics and Prebiotics as a Therapeutic Strategy to Improve Memory in a Model of Middle-Aged Rats. Front Aging Neurosci. 10, 416 (2018). ELISA, PMC6305305
||Assay Methodology: Quantitative Competitive ELISA