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GABA-B Receptor 1 Antibody (OASE00251)

100ug
$379.00
In Stock
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Conjugation Options

OASE00251-FITC Conjugated

OASE00251-HRP Conjugated

OASE00251-Biotin Conjugated

Gene Symbol:
Gabbr1
NCBI Gene Id:
81657
Official Gene Full Name:
gamma-aminobutyric acid (GABA) B receptor 1
Swissprot Id:
Q9Z0U4
Protein Accession #:
NP_112290.2
Replacement Item:
This antibody may replace item sc-116203 from Santa Cruz Biotechnology.
Host:
Mouse
Clonality:
Monoclonal
Purification:
Protein G Purified
Application:
WB, ICC
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express Gabbr1.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express Gabbr1.
Immunogen:
Fusion protein amino acids 873-977 (cytoplasmic C-terminus) of rat GABA(B)R1
Predicted Species Reactivity:
Human, Mouse, Rat
Reconstitution and Storage:
Store at -20C. Shipping in Blue Ice or 4C.
Concentration:
1mg/mL
Datasheets/Manuals:
Printable datasheet for OASE00251
Dilution:
1:1000 (WB)
Clone:
S93A-49
Isotype:
IgG1
Additional Information:
Background Info: Detects ~115kDa. No cross-reactivity against GABA(B)R2
:::
Scientific Background: GABA (?-aminobutyric acid) is the primary inhibitory neurotransmitter in the central nervous system and interacts with three different receptors: GABA(A), GABA(B) and GABA(C) receptor. The ionotropic GABA(A) and GABA(C) receptors are ligand-gated ion channels that produce fast inhibitory synaptic transmission. In contrast, the metabotropic GABA(B) receptor is coupled to G proteins that modulate slow inhibitory synaptic transmission (1). Functional GABA(B) receptors form heterodimers of GABA(B)R1 and GABA(B)R2 where GABA(B)R1 binds the ligand and GABA(B)R2 is the primary G protein contact site (2). Two isoforms of GABA(B)R1 have been cloned: GABA(B)R1a is a 130 kD protein and GABA(B)R1b is a 95 kD protein (3). G proteins subsequently inhibit adenyl cylase activity and modulate inositol phospholipid hydrolysis. GABA(B) receptors have both pre- and postsynaptic inhibitions: presynaptic GABA(B) receptors inhibit neurotransmitter release through suppression of high threshold calcium channels, while postsynaptic GABA(B) receptors inhibit through coupled activation of inwardly rectifying potassium channels. In addition to synaptic inhibition, GABA(B) receptors may also be involved in hippocampal long-term potentiation, slow wave sleep and muscle relaxation (1).
:::
Certificate of Analysis: 1ug/mL was sufficient for detection of GABA(B)R1 in 20ug of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Storage Buffer:
PBS pH7.4, 50% glycerol
Target Reference:
1. Jones K.A. et al. (2000) Neuropsychopharmacology 23: 2. Duthey B. et al. (2002) J Biol Chem. 277: 3236-41.
3. Kaupmann K. et al. (1997) Nature 386: 239-46.

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