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Catalog No: OKCD01979 (Formerly GWB-KBBHK2)
Size:96WELLS
Price: $700.00
SKU
OKCD01979
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Datasheets/ManualsPrintable datasheet for G6pc ELISA Kit (Mouse) (OKCD01979)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)97-104%96-103%83-105%95-105%
EDTA Plasma (n=5)96-105%87-96%93-101%96-104%
Heparin Plasma (n=5)98-105%81-103%81-105%98-105%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glucose-6-Phosphatase, Catalytic (G6PC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glucose-6-Phosphatase, Catalytic (G6PC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glucose-6-Phosphatase, Catalytic (G6PC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Glucose-6-Phosphatase, Catalytic (G6PC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.781-50ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)98-105102
EDTA Plasma (n=5)88-9792
Heparin Plasma (n=5)81-9794
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glucose-6-Phosphatase, Catalytic (G6PC) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glucose-6-Phosphatase, Catalytic (G6PC) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-G6pc Microplate96 Wells (12 x 8 Well Strips)
G6pc Lyophilized Standard2 x 50 ng
100X Biotinylated G6pc Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity< 0.33 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Glucose-6-Phosphatase, Catalytic (G6PC). No significant cross-reactivity or interference between Glucose-6-Phosphatase, Catalytic (G6PC) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolG6pc
Gene Full Nameglucose-6-phosphatase, catalytic
Alias SymbolsAW107337;G6P;G6Pase;G6pc1;G6pt;Glc-6-;Glc-6-Pase;Glc-6-Pase-alpha;glucose-6-phosphatase catalytic subunit 1.
NCBI Gene Id14377
Protein NameGlucose-6-phosphatase
Description of TargetHydrolyzes glucose-6-phosphate to glucose in the endoplasmic reticulum. Forms with the glucose-6-phosphate transporter (SLC37A4/G6PT) the complex responsible for glucose production through glycogenolysis and gluconeogenesis. Hence, it is the key enzyme in homeostatic regulation of blood glucose levels.
Uniprot IDP35576
Protein Accession #NP_032087.2
Nucleotide Accession #NM_008061.4
Protein Size (# AA)357
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