G6PC ELISA Kit (Human) (OKDD00277)

Catalog No: OKDD00277 (Formerly GWB-KBBBE9)

Size:96 Wells

Human G6PC ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked immunosorbent assay
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~4 Hours
• ELISA Kit Principle: Aviva Systems Biology G6PC ELISA Kit (Human) (OKDD00277) is based on standard sandwich enzyme-linked immuno-sorbent assay methodology. An antibody for G6PC has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for G6PC is added, incubated and removed. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample G6PC captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility:

  Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level G6PC were tested 20 times on one plate, respectively.

  Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level G6PC were tested on 3 different plates, 8 replicates in each plate.

  Intra-Assay: CV<10%

  Inter-Assay: CV<12%

• ELISA Kit Component:
  • Anti-G6PC Microplate: 96 Wells (12 x 8 Well Strips)
  • G6PC Lyophlized Standard: 2 x 10 ng
  • Standard Diluent: 1 x 20 mL
  • 100X G6PC Biotinylated Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Detection Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Detection Conjugate Diluent: 1 x 12 mL
  • 10X Sample Diluent: 1 x 30 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates, Cell lysates
• Sensitivity: < 0.063 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of G6PC. No significant cross-reactivity or interference between G6PC and analogues was observed.
• Assay Info: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: G6PC1
• Gene Full Name: glucose-6-phosphatase catalytic subunit 1
• Alias Symbols: G6Pase;G-6-Pase;G6Pase-alpha;G6PC;G6PT;glucose-6-phosphatase alpha;glucose-6-phosphatase catalytic subunit 1;GSD1;GSD1a.
• NCBI Gene Id: 2538
• Protein Name: Glucose-6-phosphatase
• Description of Target: Hydrolyzes glucose-6-phosphate to glucose in the endoplasmic reticulum. Forms with the glucose-6-phosphate transporter (SLC37A4/G6PT) the complex responsible for glucose production through glycogenolysis and gluconeogenesis. Hence, it is the key enzyme in homeostatic regulation of blood glucose levels.
• Uniprot ID: P35575
• Protein Accession #: NP_000142.2
• Nucleotide Accession #: NM_000151.3
• Protein Size (# AA): 357
• Molecular Weight: 40 kDa