G6PC ELISA Kit (Human) (OKCD09142)

Catalog No: OKCD09142

Size:96WELLS

G6PC ELISA Kit (Human) (OKCD09142) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glucose-6-Phosphatase, Catalytic (G6PC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glucose-6-Phosphatase, Catalytic (G6PC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glucose-6-Phosphatase, Catalytic (G6PC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Glucose-6-Phosphatase, Catalytic (G6PC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glucose-6-Phosphatase, Catalytic (G6PC) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glucose-6-Phosphatase, Catalytic (G6PC) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • G6PC Microplate: 96 Wells (12 x 8 Well strips)
  • G6PC Lyophilized Standard: 2
  • 100X G6PC HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.055ng/mL

Target Info

• Gene Symbol: G6PC1
• Gene Full Name: glucose-6-phosphatase catalytic subunit 1
• Alias Symbols: G6Pase;G-6-Pase;G6Pase-alpha;G6PC;G6PT;glucose-6-phosphatase alpha;glucose-6-phosphatase catalytic subunit 1;GSD1;GSD1a.
• NCBI Gene Id: 2538
• Protein Name: Glucose-6-phosphatase
• Description of Target: Hydrolyzes glucose-6-phosphate to glucose in the endoplasmic reticulum. Forms with the glucose-6-phosphate transporter (SLC37A4/G6PT) the complex responsible for glucose production through glycogenolysis and gluconeogenesis. Hence, it is the key enzyme in homeostatic regulation of blood glucose levels.
• Uniprot ID: P35575
• Protein Accession #: NP_000142.2
• Nucleotide Accession #: NM_000151.3