G6PC Antibody - middle region (OAAB02641)

Catalog No: OAAB02641 (Formerly GWB-WKT3TF)

Size:400UL

Western blot analysis of G6PC Antibody (Center) in K562 cell lysates (35ug/lane).G6PC (arrow) was detected using the purified Pab.

G6PC is strongly supported by BioGPS gene expression data to be expressed in K562

Anti-G6PC Antibody (middle region) at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 40 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

G6PC Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the G6PC Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

G6PC Antibody (middle region) (Cat. # OAAB02641) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: Flow cytometry, Immunohistochemistry-Paraffin, Western blot
  Cellular Location: Endoplasmic reticulum membrane; Multi-pass membrane protein.
• Reconstitution and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This G6PC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 123-149 amino acids from the Central region of human G6PC.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Varies by lot. See vial for concentration.
• Sample Type Confirmation:

  G6PC is strongly supported by BioGPS gene expression data to be expressed in K562

• Intended Use: For research use only and not for use in diagnostic or therapeutic procedures.
  
• Application Info: WB: 1:1000
  IHC-P: 1:50~100
  FC: 1:10~50
• Reference: Tu, E., et al. Hum. Pathol. 41(3):392-400(2010)
  Samuel, V.T., et al. Proc. Natl. Acad. Sci. U.S.A. 106(29):12121-12126(2009)
  Hu, C., et al. Diabetologia 52(3):451-456(2009)

Target Info

• Gene Symbol: G6PC1
• Gene Full Name: glucose-6-phosphatase catalytic subunit 1
• Alias Symbols: G6Pase;G-6-Pase;G6Pase-alpha;G6PC;G6PT;glucose-6-phosphatase alpha;glucose-6-phosphatase catalytic subunit 1;GSD1;GSD1a.
• NCBI Gene Id: 2538
• Protein Name: NP_000142.2, NP_001257326.1, P35575
• Description of Target: Glucose-6-phosphatase, glucose-6-phosphatase isoform 1 [Homo sapiens], glucose-6-phosphatase isoform 2 [Homo sapiens]
• Uniprot ID: https://www.uniprot.org/uniprot/P35575
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/NP_000142.2,NP_001257326.1
• Molecular Weight: 40484