Catalog No: OCOA12452
Size:20UG
Price: $179.00
SKU
OCOA12452
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G3BP1 Protein Lysate (OCOA12452)

Datasheets/ManualsPrintable datasheet for G3BP1 Protein Lysate (OCOA12452)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionG3BP1 HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolG3BP1
Gene Full NameG3BP stress granule assembly factor 1
Alias SymbolsATP-dependent DNA helicase VIII;DNA helicase VIII;G3BP;G3BP-1;GAP binding protein;GAP SH3 domain-binding protein 1;GTPase activating protein (SH3 domain) binding protein 1;HDH-VIII;ras GTPase-activating protein-binding protein 1;RasGAP-associated endoribonuclease G3BP;Ras-GTPase-activating protein SH3-domain-binding protein.
NCBI Gene Id10146
Protein NameNM_005754, NP_005745, Q13283
Description of TargetHomo sapiens G3BP stress granule assembly factor 1 (G3BP1), transcript variant 1, mRNA, Ras GTPase-activating protein-binding protein 1, ras GTPase-activating protein-binding protein 1 [Homo sapiens]
Uniprot IDhttps://www.uniprot.org/uniprot/Q13283
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_005745
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_005754
Molecular Weight52.2 kDa
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