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Datasheets/Manuals | Printable datasheet for anti-G3BP1 (OAAJ02714) antibody |
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Predicted Species Reactivity | Human, Mouse |
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Product Format | Liquid. PBS pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Clonality | Polyclonal |
Isotype | IgG |
Host | Rabbit |
Application | WB, IHC |
:: | Function: May be a regulated effector of stress granule assembly. Phosphorylation-dependent sequence-specific endoribonuclease in vitro. Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR. ATP- and magnesium-dependent helicase. Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends. Unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency. Acts unidirectionally by moving in the 5' to 3' direction along the bound single-stranded DNA. Subcellular Location: Cytosol; Nucleus; Plasma Membrane Tissue Specificity: Ubiquitous |
Reconstitution and Storage | Store at -20C. Stable for 12 months from date of receipt. |
Immunogen | A synthesized peptide derived from human G3BP-1 around the phosphorylation site of Serine 232 |
Purification | The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns. |
Concentration | 1 mg/ml |
Target Post-Translational Modification | Phosphorylated exclusively on serine residues. Hyperphosphorylated in quiescent fibroblasts. Hypophosphorylation leads to a decrease in endoribonuclease activity (By similarity). RASA1-dependent phosphorylation of Ser-149 induces a conformational change that prevents self-association. Dephosphorylation after HRAS activation is required for stress granule assembly. Ser-149 phosphorylation induces partial nuclear localization.Arg-435 is dimethylated, probably to asymmetric dimethylarginine. |
Specificity | Phospho-G3BP-1 (Ser232) antibody detects endogenous levels of G3BP-1 only when phosphorylated at Serine 232 |
Application Info | WB: 1:500~2000 IHC: 1:50~200 |
Gene Symbol | G3BP1 |
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Gene Full Name | GTPase activating protein (SH3 domain) binding protein 1 |
Alias Symbols | G3BP, HDH-VIII |
NCBI Gene Id | 10146 |
Description of Target | G3BP-1 an hnRNA-binding protein and endoribonuclease that participates in the Ras signal transduction pathway. A regulated effector of stress granule (SG) assembly. SGs are involved in mRNA sorting in the storing of untranslated mRNAs. |
Uniprot ID | Q13283 |
Molecular Weight | 60 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "G3BP1 Antibody (Phospho-Ser232) (OAAJ02714)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Mouse".
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How long will it take to receive "G3BP1 Antibody (Phospho-Ser232) (OAAJ02714)"?
This item is available "Domestic: within 2-3 week delivery | International: 2-3 weeks".
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What buffer format is "G3BP1 Antibody (Phospho-Ser232) (OAAJ02714)" provided in?
This item is provided in "Liquid. PBS pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "G3BP1 Antibody (Phospho-Ser232) (OAAJ02714)"?
This target may also be called "G3BP, HDH-VIII" in publications.
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What is the shipping cost for "G3BP1 Antibody (Phospho-Ser232) (OAAJ02714)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "G3BP1 Antibody (Phospho-Ser232) (OAAJ02714)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "G3BP1 Antibody (Phospho-Ser232) (OAAJ02714)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "60 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "G3BP1 Antibody (Phospho-Ser232) (OAAJ02714)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "G3BP1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "G3BP1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "G3BP1"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "G3BP1"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "G3BP1"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "G3BP1"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.