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G-CSF ELISA Kit (Mouse) : 96 Wells (OKAG00074)

96 Wells
$479.00
In Stock
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Application:
ELISA
Gene Symbol:
CSF3
Official Gene Full Name:
colony stimulating factor 3 (granulocyte)
NCBI Gene Id:
12985
Alias Symbols:
Csfg, G-CSF, Granulocyte colony-stimulating factor, MGI-IG
Description of Target:
Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.
Protein Name:
Granulocyte colony-stimulating factor
Swissprot Id:
P09920
Protein Accession #:
NP_034101.1
Nucleotide Accession #:
NM_009971.1
Predicted Species Reactivity:
Mouse
Sample Type:
Cell lysates, sera and plasma
Sensitivity:
32 pg/mL
Kit Range:
32-2,000 pg/mL
Kit Principle:
The Aviva Murine G-CSF ELISA Kit contains the components necessary for quantitative determination of natural or recombinant mG-CSF concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on the Murine G-CSF cytokine while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3’, 5, 5’-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration.
Kit Component:
ComponentsAmount
Capture Antibody Coated Microplate96 Wells (12 x 8)
Ready-to-Use Streptavidin-HRP12 mL
Ready-to-Use Substrate12 mL
Stop Solution12 mL
Wash Buffer (15x)50 mL
Protein Standard Diluent12 mL
Sample Diluent12 mL
Detection Antibody Diluent12 mL
Biotin-Labeled Detection AntibodyLyophilized
Protein StandardLyophilized (100 ng)
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express CSF3.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express CSF3.
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Reconstitution and Storage:
Store as indicated in product manual.
Product Format:
1 x 96-Well Plate or 12 x 8-Well Strips
Specificity:
The Murine G-CSF ELISA is capable of recognizing both recombinant and naturally produced Murine G-CSF proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference.
• Human: CT-1, G-CSF, GM-CSF, IL-3, IL-6, Leptin, M-CSF, SCF
• Murine: CT-1, GM-CSF, IL-3, IL-6, Leptin, M-CSF, SCF
• Rat: GM-CSF, IL-3β, IL-6, Leptin
Assay Info:
Quantitative Colorimentric Sandwich ELISA

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