- Official Gene Full Name:
- colony stimulating factor 3 (granulocyte)
- NCBI Gene Id:
- Alias Symbols:
- Csfg, G-CSF, Granulocyte colony-stimulating factor, MGI-IG
- Description of Target:
- Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.
- Protein Name:
- Granulocyte colony-stimulating factor
- Sample Type:
- Cell lysates, sera and plasma
- 32 pg/mL
- Kit Range:
- 32-2,000 pg/mL
- Kit Principle:
- The Aviva Murine G-CSF ELISA Kit contains the components necessary for quantitative determination of natural or recombinant mG-CSF concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on the Murine G-CSF cytokine while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3’, 5, 5’-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration.
- Kit Component:
|Capture Antibody Coated Microplate||96 Wells (12 x 8)|
|Ready-to-Use Streptavidin-HRP||12 mL|
|Ready-to-Use Substrate||12 mL|
|Stop Solution||12 mL|
|Wash Buffer (15x)||50 mL|
|Protein Standard Diluent||12 mL|
|Sample Diluent||12 mL|
|Detection Antibody Diluent||12 mL|
|Biotin-Labeled Detection Antibody||Lyophilized|
|Protein Standard||Lyophilized (100 ng)|
- Kit Detection Method:
- Colorimetric, OD450 nm
- Tissue Tool:
- Find tissues and cell lines supported by DNA array analysis to express CSF3.
- RNA Seq:
- Find tissues and cell lines supported by RNA-seq analysis to express CSF3.
- Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
- Reconstitution and Storage:
- Store as indicated in product manual.
- Product Format:
- 1 x 96-Well Plate or 12 x 8-Well Strips
- The Murine G-CSF ELISA is capable of recognizing both recombinant and naturally produced Murine G-CSF proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference.
• Human: CT-1, G-CSF, GM-CSF, IL-3, IL-6, Leptin, M-CSF, SCF
• Murine: CT-1, GM-CSF, IL-3, IL-6, Leptin, M-CSF, SCF
• Rat: GM-CSF, IL-3β, IL-6, Leptin
- Assay Info:
- Quantitative Colorimentric Sandwich ELISA