FZR1 Antibody (OACA06501)

Catalog No: OACA06501

Immunohistochemistry of paraffin-embedded human testis tissue at dilution of 1:100

Western blot
All lanes: FZR1 antibody at 6ug/ml
Lane 1: Hela whole cell lysate
Lane 2: Jurkat whole cell lysate
Lane 3: 293T whole cell lysate
Secondary
Goat polyclonal to Rabbit IgG at 1/1,0000 dilution
Predicted band size: 56,55,45 kDa
Observed band size: 55 kDa

Western blot
All lanes: FZR1 antibody at 6ug/ml
Lane 1: Hela whole cell lysate
Lane 2: Jurkat whole cell lysate
Lane 3: 293T whole cell lysate
Secondary
Goat polyclonal to Rabbit IgG at 1/1,0000 dilution
Predicted band size: 56,55,45 kDa
Observed band size: 55 kDa

Immunohistochemistry of paraffin-embedded human testis tissue at dilution of 1:100

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Immunohistochemistry|Western blot
• Reconstitution and Storage: -20°C or -80°C
• Immunogen: Recombinant Human Fizzy-related protein homolog protein (1-493AA)
• Purification: Protein G Purified
• Preservative: 0.03% Proclin 300
• Formulation: 50% Glycerol, 0.01M PBS, PH 7.4
• Reference: [1]"Male germ cell-associated kinase is overexpressed in prostate cancer cells and causes mitotic defects via deregulation of APC/C(CDH1)." Wang L.Y., Kung H.J. Oncogene 31:2907-2918(2011). [2]"SIRT2 maintains genome integrity and suppresses tumorigenesis through regulating APC/C activity." Kim H.S., Vassilopoulos A., Wang R.H., Lahusen T., Xiao Z., Xu X., Li C., Veenstra T.D., Li B., Yu H., Ji J., Wang X.W., Park S.H., Cha Y.I., Gius D., Deng C.X. Cancer Cell 20:487-499(2010). [3]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2009).

Target Info

• Gene Symbol: FZR1
• Gene Full Name: fizzy and cell division cycle 20 related 1
• Alias Symbols: CDC20 homolog 1;CDC20C;CDC20-like 1b;CDC20-like protein 1;CDH1;cdh1/Hct1 homolog;fizzy/cell division cycle 20 related 1;fizzy-related protein homolog;FZR;FZR2;HCDH;HCDH1.
• NCBI Gene Id: 51343
• Protein Name: Fizzy-related protein homolog
• Description of Target: Substrate-specific adapter for the anaphase promoting complex/cyclosome (APC/C) E3 ubiquitin-protein ligase complex. Associates with the APC/C in late mitosis, in replacement of CDC20, and activates the APC/C during anaphase and telophase. The APC/C remains active in degrading substrates to ensure that positive regulators of the cell cycle do not accumulate prematurely. At the G1/S transition FZR1 is phosphorylated, leading to its dissociation from the APC/C. Following DNA damage, it is required for the G2 DNA damage checkpoint: its dephosphorylation and reassociation with the APC/C leads to the ubiquitination of PLK1, preventing entry into mitosis. Acts as an adapter for APC/C to target the DNA-end resection factor RBBP8/CtIP for ubiquitination and subsequent proteasomal degradation. Through the regulation of RBBP8/CtIP protein turnover, may play a role in DNA damage response, favoring DNA double-strand repair through error-prone non-homologous end joining (NHEJ) over error-free, RBBP8-mediated homologous recombination (HR) (PubMed:25349192).
• Uniprot ID: Q9UM11
• Protein Accession #: NP_001129669
• Nucleotide Accession #: NM_001136197