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Datasheets/Manuals | Printable datasheet for FZD8 Antibody (OAAF04821) |
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Predicted Species Reactivity | Human|Mouse|Rat |
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Product Format | Liquid |
Clonality | Polyclonal |
Host | Rabbit |
Application | Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Western blot |
Reconstitution and Storage | -20°C |
Immunogen | The antiserum was produced against synthesized peptide derived from human FZD8. |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Specificity | FZD8 antibody detects endogenous levels of total FZD8 protein. |
Formulation | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Dilution | The optimal working dilution should be determined by the end user. |
Application Info | WB: 1:500~1:3000; IF: 1:100~1:500; ELISA: 1:5000 |
Reference | Saitoh T., Int. J. Oncol. 18:991-996(2001). Deloukas P., Nature 429:375-381(2004). |
Gene Symbol | FZD5|FZD8 |
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Gene Full Name | frizzled class receptor 5|frizzled class receptor 8 |
Alias Symbols | C2orf31;frizzled 5, seven transmembrane spanning receptor;frizzled 8, seven transmembrane spanning receptor;frizzled family receptor 5;frizzled family receptor 8;frizzled homolog 8;frizzled-5;frizzled-8;fz-5;FZ-8;fzE5;HFZ5;hFZ8;seven-transmembrane receptor frizzled-5;Wnt receptor. |
NCBI Gene Id | 7855|8325 |
Protein Name | Frizzled-5|Frizzled-8 |
Description of Target | Receptor for Wnt proteins (PubMed:9054360, PubMed:10097073, PubMed:20530549). Can activate WNT2, WNT10B, WNT5A, but not WNT2B or WNT4 (in vitro); the in vivo situation may be different since not all of these are known to be coexpressed (By similarity). In neurons, activation of WNT7A promotes formation of synapses (PubMed:20530549). Functions in the canonical Wnt/beta-catenin signaling pathway. The canonical Wnt/beta-catenin signaling pathway leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes (By similarity). A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues (Probable). Plays a role in yolk sac angiogenesis and in placental vascularization (By similarity).|Receptor for Wnt proteins. Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor-ligand complexes into ribosome-sized signalosomes. The beta-catenin canonical signaling pathway leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Coreceptor along with RYK of Wnt proteins, such as WNT1. |
Uniprot ID | Q13467|Q9H461 |
Protein Accession # | NP_114072.1 |
Molecular Weight | 73 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "FZD8 Antibody (OAAF04821)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Mouse|Rat".
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How long will it take to receive "FZD8 Antibody (OAAF04821)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "FZD8 Antibody (OAAF04821)" provided in?
This item is provided in "Liquid".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "FZD8 Antibody (OAAF04821)"?
This target may also be called "C2orf31;frizzled 5, seven transmembrane spanning receptor;frizzled 8, seven transmembrane spanning receptor;frizzled family receptor 5;frizzled family receptor 8;frizzled homolog 8;frizzled-5;frizzled-8;fz-5;FZ-8;fzE5;HFZ5;hFZ8;seven-transmembrane receptor frizzled-5;Wnt receptor." in publications.
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What is the shipping cost for "FZD8 Antibody (OAAF04821)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "FZD8 Antibody (OAAF04821)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "FZD8 Antibody (OAAF04821)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "73 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "FZD8 Antibody (OAAF04821)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "FZD5|FZD8"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "FZD5|FZD8"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "FZD5|FZD8"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "FZD5|FZD8"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "FZD5|FZD8"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "FZD5|FZD8"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.