Size:20 ug
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FZD4 Protein Lysate (OCOA01970)

Catalog#: OCOA01970
Domestic: within 1 week delivery | International: 1 week
More Information
Predicted Species Reactivity Human
Product Format Lyophilized
Host HEK293T
Additional Information Components: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
:: Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and Storage The lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
Datasheets/Manuals Printable datasheet for OCOA01970
Gene Symbol FZD4
Alias Symbols FZD4, CD344, EVR1, FEVR, Fz-4, Fz4, FZD4S, FzE4, GPCR, hFz4
NCBI Gene Id 8322
Protein Accession # NP_036325
Nucleotide Accession # NM_012193
Molecular Weight 60.3 kDa
Tissue Tool Find tissues and cell lines supported by DNA array analysis to express FZD4.
RNA Seq Find tissues and cell lines supported by RNA-seq analysis to express FZD4.
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