FZD1 Antibody (OAAF04887)

Catalog No: OAAF04887 (Formerly GWB-ASF286)

Size:100 ug

Western blot analysis of extracts from Jurkat cells, using FZD1 Antibody. The lane on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Western blot
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human FZD1.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Specificity: FZD1 antibody detects endogenous levels of total FZD1 protein.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: WB: 1:500~1:3000; ELISA: 1:5000
• Reference: Gazit A., Oncogene 18:5959-5966(1999).
  Sagara N., Biochem. Biophys. Res. Commun. 252:117-122(1998).
  Hillier L.W., Nature 424:157-164(2003).

Target Info

• Gene Symbol: FZD1
• Gene Full Name: frizzled class receptor 1
• Alias Symbols: frizzled 1, seven transmembrane spanning receptor;frizzled family receptor 1;frizzled homolog 1;frizzled, Drosophila, homolog of, 1;frizzled-1;fz-1;fzE1;hFz1;Wnt receptor.
• NCBI Gene Id: 8321
• Protein Name: Frizzled-1
• Description of Target: Receptor for Wnt proteins (PubMed:10557084). Activated by WNT3A, WNT3, WNT1 and to a lesser extent WNT2, but apparently not by WNT4, WNT5A, WNT5B, WNT6, WNT7A or WNT7B (PubMed:10557084). Contradictory results showing activation by WNT7B have been described for mouse (By similarity). Functions in the canonical Wnt/beta-catenin signaling pathway (PubMed:10557084). The canonical Wnt/beta-catenin signaling pathway leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes (PubMed:10557084). A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues (Probable).
• Uniprot ID: Q9UP38
• Protein Accession #: NP_003496.1
• Molecular Weight: 71 kDa